FIGURE 3. Functional and expression analysis of F3'h.
From the following article:
Genetic changes associated with floral adaptation restrict future evolutionary potential
Rebecca A. Zufall and Mark D. Rausher
Nature 428, 847-850(22 April 2004)
doi:10.1038/nature02489
a, Thin-layer chromatogram illustrating that F3'h from both I. purpurea and I. quamoclit metabolize dihydrokaempferol (DHK) to produce dihydroquercitin (DHQ) in in vitro assays. b, Mean production of DHQ in in vitro enzyme assays. Bars are one standard error. c, Accumulation of anthocyanins in F3'h-null A. thaliana seedlings carrying the transgene F3'h from (left) I. purpurea (substantial accumulation) and (right) I. quamoclit (no visible accumulation). d, Anthocyanin gene expression patterns. F3'h is highlighted with a red label. Left panel, spotted I. purpurea genes probed with I. purpurea cDNA. All genes, including F3'h, exhibit substantial expression. Right panel, left lane contains spotted genes from I. purpurea, right lane genes from I. quamoclit. Note that only Dfr, Ans and F3'h have been spotted (each gene twice), because the other anthocyanin genes have not been cloned from I. quamoclit. Both lanes of right panel were probed with I. quamoclit cDNA.
