FIGURE 1. hCDC4 mutations in colorectal cancers and adenomas.

a, Location (arrows) and frequency (asterisks) of mutations in hCDC4. Black arrows indicate nonsense mutations, red arrows indicate missense mutations, black asterisks indicate the number of mutations found in cancers, green asterisks show mutations found in adenomas. b, Western blotting for Myc (Myc-tagged CDC4) and cyclin E after immunoprecipitation with an antibody against Myc. Cont, untransfected cells; WT, cells co-transfected with cyclin E and Myc-tagged wild-type CDC4; Mut, cells co-transfected with cyclin E and Myc-tagged mutant CDC4 (Arg465Cys). c, d, Three-dimensional views of yeast CDC4 bound to human cyclin E^8,9. CDC4 is shown in yellow, cyclin E in blue. Amino acids in CDC4 that align with residues affected by missense mutations in hCDC4 are labelled in red. c, Longitudinal view. d, View down the z-axis.