Supplementary information
From the following article:
Direct interaction of geminin and Six3 in eye development
Filippo Del Bene, Kristin Tessmar-Raible & Joachim Wittbrodt
Nature 427, 745-749(19 February 2004)
doi:10.1038/nature02292
Supplementary Figure 2
Mapping of the interacting domains of Geminin.
Supplementary Figure 3
Luciferase assay indicates inhibitory effect of Geminin on Six3 transcriptional activity.
Supplementary Figure 4
Geminin overxpression results in reduction of anterior neural marker expression.
Supplementary Figure 5
a) Antisense morpholino oligonucleotide blocks translation of medaka Geminin; b) addition of MoGFP, MoMiss or MoGem at 0.1mM to in vitro transcription/translation system reactions; c) Western Blot analysis of gemUTR-GFP expression in injected embryos.
Supplementary Figure 6
Geminin depletion and Six3 overexpression synergize in inducing retinal hyperplasia.
Supplementary Table 1
Concentration dependent effect upon injection of geminin mRNA and phenotypic rescue after coinjection with Six3 or Six3W216R mRNAs.
Supplementary Table 2
Morpholino injection experiments.
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