FIGURE 3. Mature single-positive (SP) thymocytes upregulate S1P1 and respond to S1P in an S1P1-dependent manner.
From the following article:
Lymphocyte egress from thymus and peripheral lymphoid organs is dependent on S1P receptor 1
Mehrdad Matloubian, Charles G. Lo, Guy Cinamon, Matthew J. Lesneski, Ying Xu, Volker Brinkmann, Maria L. Allende, Richard L. Proia & Jason G. Cyster
Nature 427, 355-360(22 January 2004)
doi:10.1038/nature02284
a, b, Chemotactic response of different thymocyte populations to S1P. The percentage of input cells of the indicated population that migrated to S1P is shown with the error bars representing the range of response for duplicate samples. c, Quantitative PCR analysis of S1P receptors and CCR7 in sorted thymocyte subsets, expressed as relative amount of indicated messenger RNA normalized to HPRT. Representative of three experiments. DP, double positive. d, e, Chemotaxis of S1P1-/- and S1P1+/+ mature (l-selectinhi) CD4 single-positive and CD8 single-positive thymocytes to S1P. The percentage of the input cell population that responded to S1P is shown in duplicate and the lines are drawn through the mean values. Inset shows chemotactic response of S1P1+/+ and S1P1-/- CD4 single-positive and CD8 single-positive thymocytes to 1 
g ml-1 CCL21/SLC.
