Supplementary information

From the following article:

Vernalization requires epigenetic silencing of FLC by histone methylation

Ruth Bastow, Joshua S. Mylne, Clare Lister, Zachary Lippman, Robert A. Martienssen and Caroline Dean

Nature 427, 164-167(8 January 2004)

doi:10.1038/nature02269

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Supplementary Figure 1: H3 dimethyl K4 and H3 dimethyl K9 histone modifications at FLC are associated with vernalization and are dependent on the VRN genes.

Supplementary Figure 2: The H3 dimethyl K27 histone modification at FLC is associated with vernalization and is dependent on VRN2, but not VRN1.

Supplementary Figure 3: Primers used for ChIP analysis.

Supplementary Figure 4: PCR analysis to show the amplification efficiency of all primer pairs used in the chromatin immunoprecipitation analysis.

Supplementary Figure Legends

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