FIGURE 3. RT–PCR detection of germ-cell-specific genes in SSEA1+ cells isolated from developing EBs.
From the following article:
Derivation of embryonic germ cells and male gametes from embryonic stem cells
Niels Geijsen, Melissa Horoschak, Kitai Kim, Joost Gribnau, Kevin Eggan and George Q. Daley
Nature 427, 148-154(8 January 2004)
doi:10.1038/nature02247
a, Expression of Oct4 during EB development (left panel) and in SSEA1+ and SSEA1- fractions derived by immunomagnetic sorting. Flow cytometric analysis demonstrates >90% purity in the SSEA1+ fraction and >99% purity of the SSEA1- fraction (data not shown). b, Expression of Tex14, Rnh2, Piwil2 and Dazl assayed by RT–PCR. Actin served as a control. c, Immunohistochemistry of germ cell and haematopoietic development in day 7 EBs. EBs were cryosectioned and stained with CD41–fluorescein isothiocyanate (green) to mark early haematopoietic cells and SSEA1–PE (red) to visualize germ cell development.
