FIGURE 3. CD19–Cre-mediated deletion of Mcl-1 blocks B-cell development.

a, The percentage of bone marrow pre-pro-B (CD43⁺B220⁺, right gate), pro-B (CD43^-B220⁺, middle gate) and pre-B (CD43^-B220⁺, left gate) subsets as determined by flow cytometry for f/wt and CD19–Cre^f/null genotypes. b, Total B-cell progenitors (mean s.e.m.) recovered from the bone marrow of f/wt and CD19–Cre^f/null genotypes at 8 weeks of age. c, Genomic DNA from sorted cells representing Hardy subsets (A, B, C, D) of B-cell progenitors from CD19–Cre^f/wt mice was analysed by PCR to detect the Mcl-1 allele. d, Percentage apoptosis of pro-B (CD43^-B220⁺) cells from f/wt or CD19–Cre^f/null mice was assessed with Annexin-V. Histograms are representative of three mice analysed at 8 weeks of age.