FIGURE 3. PTH administration expands HSCs.
From the following article:
Osteoblastic cells regulate the haematopoietic stem cell niche
L. M. Calvi, G. B. Adams, K. W. Weibrecht, J. M. Weber, D. P. Olson, M. C. Knight, R. P. Martin, E. Schipani, P. Divieti, F. R. Bringhurst, L. A. Milner, H. M. Kronenberg and D. T. Scadden
Nature 425, 841-846(23 October 2003)
doi:10.1038/nature02040
a, PTH-treated wild-type BM-SCs reproduce the col1-caPPR effect (SP, stromal preparation; CM, culture maintenance). b, Increased numbers of alkaline-phosphatase-positive BM-SCs (red) in PTH-treated cultures (n = 4 in both groups). c, PTH-mediated HSC expansion was blocked by the addition of 
-secretase inhibitor to LTC-IC assays. d, PTH-treated mice (n = 8) had increased Lin-Sca-1+c-Kit+ cells (mock-injected mice, n = 7) (mean absolute number per hind limb: PTH-treated, 211,000 
12,000; mock-treated, 139,000 29,000). e, PTH-treated mice had increased LTC-IC frequency. f, Bone marrow cells from PTH-treated mice had increased ability to reconstitute secondary recipients as shown by flow cytometry for CD45.2 (n = 5 in each group). g, Unchanged CFU-C frequency (n = 5 in each group).
