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Letters to Nature

Nature 425, 393-397 (25 September 2003) | doi:10.1038/nature01853; Received 18 February 2003; Accepted 5 June 2003

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Cytosolic pH regulates root water transport during anoxic stress through gating of aquaporins

Colette Tournaire-Roux1, Moira Sutka1, Hélène Javot1, Elisabeth Gout2, Patricia Gerbeau1,3, Doan-Trung Luu1, Richard Bligny2 & Christophe Maurel1

  1. Biochimie et Physiologie Moléculaire des Plantes, Centre National de la Recherche Scientifique (Unité Mixte de Recherche 5004), Institut National de la Recherche Agronomique, Université Montpellier 2 et Ecole Nationale d'Agronomie, Place Viala, F-34060 Montpellier cedex 1, France
  2. Physiologie Cellulaire Végétale, Commissariat à l'Energie Atomique, Rue des Martyrs, F-38054 Grenoble cedex 9, France
  3. Present address: Phytobiologie Cellulaire, Université de Bourgogne, BP 47 870, F-21078 Dijon cedex, France

Correspondence to: Christophe Maurel1 Email: maurel@ensam.inra.fr

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Flooding of soils results in acute oxygen deprivation (anoxia) of plant roots during winter in temperate latitudes, or after irrigation1, and is a major problem for agriculture. One early response of plants to anoxia and other environmental stresses is downregulation of water uptake due to inhibition of the water permeability (hydraulic conductivity) of roots (Lpr)2, 3, 4, 5. Root water uptake is mediated largely by water channel proteins (aquaporins) of the plasma membrane intrinsic protein (PIP) subgroup6, 7, 8. These aquaporins may mediate stress-induced inhibition of Lpr2, 4, 9 but the mechanisms involved are unknown. Here we delineate the whole-root and cell bases for inhibition of water uptake by anoxia and link them to cytosol acidosis. We also uncover a molecular mechanism for aquaporin gating by cytosolic pH. Because it is conserved in all PIPs, this mechanism provides a basis for explaining the inhibition of Lpr by anoxia and possibly other stresses. More generally, our work opens new routes to explore pH-dependent cell signalling processes leading to regulation of water transport in plant tissues or in animal epithelia10.

  1. Biochimie et Physiologie Moléculaire des Plantes, Centre National de la Recherche Scientifique (Unité Mixte de Recherche 5004), Institut National de la Recherche Agronomique, Université Montpellier 2 et Ecole Nationale d'Agronomie, Place Viala, F-34060 Montpellier cedex 1, France
  2. Physiologie Cellulaire Végétale, Commissariat à l'Energie Atomique, Rue des Martyrs, F-38054 Grenoble cedex 9, France
  3. Present address: Phytobiologie Cellulaire, Université de Bourgogne, BP 47 870, F-21078 Dijon cedex, France

Correspondence to: Christophe Maurel1 Email: maurel@ensam.inra.fr