FIGURE 3. Expression pattern and subcellular location of CAS.
From the following article:
A cell surface receptor mediates extracellular Ca2+ sensing in guard cells
Shengcheng Han, Ruhang Tang, Lisa K. Anderson, Todd E. Woerner and Zhen-Ming Pei
Nature 425, 196-200(11 September 2003)
doi:10.1038/nature01932
a, Northern blot analysis of CAS expression in Arabidopsis. Ribosomal RNA was used as a loading control. b, CAS promoter::GUS expression in guard cells. c, Plasma membrane localization of CAS–GFP fusion protein in onion epidermal cells transiently expressing a 35S::CAS–GFP construct with the 35S::GFP as a control. d, Membrane-associated (M-p) and soluble protein (S-p) from leaves was loaded, stained with Coomassie blue (left), and blotted with anti-CAS antibodies (right). e, CAS protein localizes to the cell surface of guard cells. CAS protein was detected using anti-CAS antibodies. Inset shows one enlarged stoma.
