FIGURE 1. Maximum-likelihood tree of RdRp sequences from environmental amplicons and representative viruses from picorna-like virus families.

(See Methods for complete virus names). Viruses from the Potyviridae, which contain RdRp sequences from a different lineage⁵, were used as an outgroup. Family names and group letters are shown. Environmental amplicons from coastal British Columbia are labelled by a two- or three-letter station designation, month, year, group sequence number and GenBank database accession number (SSSMYY-AA, see also Supplementary Information). TREE-PUZZLE support values are shown for relevant nodes in boldface followed by bootstrap values based on neighbour-joining analysis. N indicates there was no corresponding node in the neighbour-joining tree. The maximum-likelihood distance scale bar indicates a distance of 0.1.