FIGURE 5. Reduced access of pH buffers to kiss-and-run vesicles.
From the following article:
Three modes of synaptic vesicular recycling revealed by single-vesicle imaging
Sunil P. Gandhi and Charles F. Stevens
Nature 423, 607-613(5 June 2003)
a, Average time course of isolated kiss-and-run events (in standard calcium concentration, see Fig. 4) shown for 10 mM and 100 mM Tris (in grey). Best fit monoexponential decays are superimposed in black. b, Plots of estimated kiss-and-run decay time across Tris concentrations. Tris slows kiss-and-run acidification, but 30% less potently than for evoked retrieval (compare slope with Fig. 3f). c, d, HEPES fails to slow kiss-and-run acidification. Averaged isolated kiss-and-run events in 10 mM and 100 mM are plotted in two upper traces in c with decay fits. Dose response is shown in d (compare with Fig. 3e). Stimulating with 900 action potentials in the presence of 100 mM HEPES before one-action-potential experiments did slow the decay time of kiss-and-run events (bottom trace in c).
