FIGURE 1. Calorie restriction and heat stress extend lifespan in a PNC1-dependent manner.

From the following article:

Nicotinamide and PNC1 govern lifespan extension by calorie restriction in Saccharomyces cerevisiae

Rozalyn M. Anderson, Kevin J. Bitterman, Jason G. Wood, Oliver Medvedik and David A. Sinclair

Nature 423, 181-185(8 May 2003)


a, Pnc1 converts nicotinamide to nicotinic acid. b, In S. cerevisiae, NAD+ is synthesized de novo from tryptophan via Bna1–6 or recycled from nicotinamide. c, Average lifespan on 2.0% (w/v) glucose: wild type, 21.6 generations; pnc1Delta, 19.1; sir2Delta, 14.2. Average lifespan on 0.5% glucose: wild type, 32.7 generations; pnc1Delta, 18.1; sir2Delta, 14.7. d, At 30 °C, average lifespans: wild type, 19.4 generations; pnc1Delta, 18.5; sir2Delta, 12.0. At 37 °C, average lifespans: wild type, 23.4 generations; pnc1Delta, 17.5; sir2Delta, 10.6. e, Average lifespans on 2.0% glucose at 30 °C: wild type, 19.7 generations; 5 times PNC1, 36.1; sir2Delta, 14.2; 5 times PNC1sir2Delta, 15.1; pnc1Deltasir2Delta, 14.4.