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Protein analysis on a proteomic scale

Nature volume 422, pages 208–215 (2003)Cite this article

Abstract

The long-term challenge of proteomics is enormous: to define the identities, quantities, structures and functions of complete complements of proteins, and to characterize how these properties vary in different cellular contexts. One critical step in tackling this goal is the generation of sets of clones that express a representative of each protein of a proteome in a useful format, followed by the analysis of these sets on a genome-wide basis. Such studies enable genetic, biochemical and cell biological technologies to be applied on a systematic level, leading to the assignment of biochemical activities, the construction of protein arrays, the identification of interactions, and the localization of proteins within cellular compartments.

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Figure 1: Analytical versus functional protein microarrays.
Figure 2: Yeast two-hybrid approaches.
Figure 3: Principle of optical detection of protein post-translational modifications on a cell microarray.

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Acknowledgements

We thank T. Davis and E. Grayhack for comments on the manuscript. This work was supported by grants from the National Center for Research Resources and National Human Genome Research Institute of the National Institutes of Health. S.F. is an investigator of the Howard Hughes Medical Institute.

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Authors and Affiliations

  1. Department of Biochemistry and Biophysics, University of Rochester School of Medicine, Box 712, 601 Elmwood Avenue, Rochester, 14642, New York, USA

    Eric Phizicky

  2. European Molecular Biology Laboratory, Meyerhofstrasse 1, Heidelberg, 69117, Germany

    Philippe I. H. Bastiaens

  3. Department of Molecular, Cellular, and Developmental Biology, PO Box 208103

    Heng Zhu & Michael Snyder

  4. Yale University, New Haven, 06520, Connecticut, USA

    Heng Zhu & Michael Snyder

  5. Departments of Genome Sciences and Medicine, Howard Hughes Medical Institute, University of Washington, Box 357730, Seattle, 98195, Washington, USA

    Stanley Fields

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Phizicky, E., Bastiaens, P., Zhu, H. et al. Protein analysis on a proteomic scale. Nature 422, 208–215 (2003). https://doi.org/10.1038/nature01512

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