FIGURE 4. Increased cytokine production in 
7-subunit-deficient mice during endotoxaemia.
From the following article:
Nicotinic acetylcholine receptor 
7 subunit is an essential regulator of inflammation
Hong Wang, Man Yu, Mahendar Ochani, Carol Ann Amella, Mahira Tanovic, Seenu Susarla, Jian Hua Li, Haichao Wang, Huan Yang, Luis Ulloa, Yousef Al-Abed, Christopher J. Czura and Kevin J. Tracey
Nature 421, 384-388(23 January 2003)
doi:10.1038/nature01339
7-deficient mice (-/-) or age- and sex-matched wild-type mice (+/+) were injected with LPS (0.1 mg kg-1, intraperitoneally). Blood and organs were obtained either 1 h (for TNF) or 4 h (for IL-1
and IL-6) after LPS administration. Levels of TNF, IL-1 and IL-6 in serum or organs were measured with enzyme-linked immunosorbent assay (ELISA). a, TNF levels in serum; n = 10 per group; b, TNF levels in liver; n = 6 per group; c, TNF levels in spleen; n = 6 per group; d, IL-1 levels in serum; n = 8 per group; e, IL-6 levels in serum; n = 9 per group. Asterisk, P < 0.05 versus wild-type controls. Horizontal line, the mean of the group.
