FIGURE 3. The G-protein-coupled chemokine receptor Cxcr4b is required for PGC guidance in zebrafish.
From the following article:
A zebrafish homologue of the chemokine receptor Cxcr4 is a germ-cell guidance receptor
Holger Knaut, Christian Werz, Robert Geisler, The Tübingen 2000 Screen Consortium and Christiane Nüsslein-Volhard
Nature 421, 279-282(16 January 2003)
doi:10.1038/nature01338
a, b, In situ hybridization reveals that at the seven-somite stage Cxcr4b is, among other tissues15, expressed in two lateral clusters at the level of the second somite (arrows in a; the boxed area in a is magnified in b). Co-staining for Cxcr4b messenger RNA (green) and Vasa protein (red) shows that these two lateral clusters correspond to PGCs (b). c, Embryos injected at the one-cell stage with Cxcr4b anti-sense morpholinos (0.2 mM, 5'-AGTGTGCTCAAAAAGGCGCAATAAG-3') display scattered PGCs (revealed by anti-Vasa staining) at 30 h after fertilization and thus recapitulate the ody phenotype. d, Injection of mismatch Cxcr4b morpholinos (0.2 mM, 5'-AGAGTCCTGAAAAAGGCGGAAAAAG-3') has no effect on PGC migration.
