Nature 420, 224-230 (14 November 2002) | doi:10.1038/nature01257

Engineered gene circuits

Jeff Hasty1, David McMillen2 & J. J. Collins2


A central focus of postgenomic research will be to understand how cellular phenomena arise from the connectivity of genes and proteins. This connectivity generates molecular network diagrams that resemble complex electrical circuits, and a systematic understanding will require the development of a mathematical framework for describing the circuitry. From an engineering perspective, the natural path towards such a framework is the construction and analysis of the underlying submodules that constitute the network. Recent experimental advances in both sequencing and genetic engineering have made this approach feasible through the design and implementation of synthetic gene networks amenable to mathematical modelling and quantitative analysis. These developments have signalled the emergence of a gene circuit discipline, which provides a framework for predicting and evaluating the dynamics of cellular processes. Synthetic gene networks will also lead to new logical forms of cellular control, which could have important applications in functional genomics, nanotechnology, and gene and cell therapy.

  1. Department of Bioengineering, University of California San Diego, La Jolla, California 92093, USA
  2. Center for BioDynamics and Department of Biomedical Engineering, Boston University, Boston, Massachusetts 02215, USA

Correspondence to: Jeff Hasty1 (e-mail: Email: