FIGURE 1. Odorant induced activation of rI7 → M71 glomeruli detected by intrinsic signal imaging.

From the following article:

Odorant receptors instruct functional circuitry in the mouse olfactory bulb

Leonardo Belluscio, Claudia Lodovichi, Paul Feinstein, Peter Mombaerts and Lawrence C. Katz

Nature 419, 296-300(19 September 2002)



ah, Dorsal view of an adult mouse olfactory bulb imaged in vivo. All odorants were presented at 1% concentration. Red arrowheads indicate the location of the rI7 right arrow M71 glomerulus. a, Epifluorescent image of an rI7 right arrow M71 GFP-labelled lateral glomerulus (green). b, c, Octanal (Oct) and heptanal (Hept) evoke focal rI7 right arrow M71 glomerular activity (dark spots). df, Hexanal (Hex), propanal (Prop) and citral (Cit) fail to elicit rI7 right arrow M71 activity. White arrowheads in ce mark other regions activated by these odorants13. g, h, Magnified rI7 right arrow M71 glomerular region from the octanal map (b) and GFP image (a), each showing four line plots (red and green lines) averaged to determine the intensity profile. i, Comparison of normalized intensity profiles of GFP (green) and intrinsic signal (IS, red). The diameters at half-maximum peak intensity show that the intrinsic signal is 22% wider (grey area). Scale bar, 200 microm.