FIGURE 1. Outline of the mRNA-tagging technique.
From the following article:
Chromosomal clustering of muscle-expressed genes in Caenorhabditis elegans
Peter J. Roy, Joshua M. Stuart, Jim Lund and Stuart K. Kim
Nature 418, 975-979(29 August 2002)
doi:10.1038/nature01012
a, Schematic of myo-3p::flag::pab-1. The initiator methionine and stop codon are shown. Dark blue boxes show the predicted pab-1 exons, white boxes show untranslated regions, and the light blue box represents the Flag tag. b, Schematic of the muscle mRNA-tagging strain. myo-3p drives expression of Flag::PAB-1 in non-pharyngeal muscle cells (red), but not in other tissues, such as the intestine (grey). Thus, Flag::PAB-1 (blue) binds only to muscle-expressed mRNAs, and only muscle-expressed mRNAs co-immunoprecipitate with Flag::PAB-1.