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Capacitance steps and fusion pores of small and large-dense-core vesicles in nerve terminals

Nature volume 418, pages 89–92 (2002)Cite this article

Abstract

The vesicles that package neurotransmitters fall into two distinct classes, large dense-core vesicles (LDCVs) and small synaptic vesicles, the coexistence of which is widespread in nerve terminals1. High resolution capacitance recording reveals unitary steps proportional to vesicle size. Measurements of capacitance steps during LDCV and secretory granule fusion in endocrine and immune cells have provided important insights into exocytosis2,3,4; however, extending these measurements to small synaptic vesicles has proven difficult. Here we report single vesicle capacitance steps in posterior pituitary nerve terminals. These nerve terminals contain neuropeptide-laden LDCVs, as well as microvesicles. Microvesicles are similar to synaptic vesicles in size, morphology5 and molecular composition6,7,8, but their contents are unknown. Capacitance steps of two characteristic sizes, corresponding with microvesicles and LDCVs, were detected in patches of nerve terminal membrane. Both types of vesicles fuse in response to depolarization-induced Ca2+ entry. Both undergo a reversible fusion process commonly referred to as ‘kiss-and-run’, but only rarely. Fusion pores seen during microvesicle kiss-and-run have a conductance of 19 pS, 11 times smaller than LDCV fusion pores. Thus, LDCVs and microvesicles use structurally different intermediates during exocytosis.

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Figure 1: Spontaneous single-vesicle capacitance steps in nerve terminals.
Figure 2: Depolarization-induced Ca2+ entry elicits exocytosis of LDCVs and microvesicles.
Figure 3: Kiss-and-run.
Figure 4: Fusion pore analysis for kiss-and-run and non-reversing events.

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Acknowledgements

We thank M. Lindau for advice on capacitance measurements, D.L. Armstrong for suggesting the use of oil to reduce noise, and E.R. Chapman, C.-T. Wang, P.Y. Chang, G.P. Ahern and X. Han for helpful comments on the manuscript. We thank R. J. Massey of the UW Electron Microscope Facility for performing electron microscopy. This research was supported by a grant from NIH and a predoctoral fellowship from the AHA to V.A.K.

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  1. Department of Physiology and Biophysics Graduate Program, University of Wisconsin-Madison, 1300 University Avenue, Wisconsin, 53706, Madison, USA

    Vitaly A. Klyachko & Meyer B. Jackson

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  1. Vitaly A. Klyachko
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Correspondence to Meyer B. Jackson.

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Klyachko, V., Jackson, M. Capacitance steps and fusion pores of small and large-dense-core vesicles in nerve terminals. Nature 418, 89–92 (2002). https://doi.org/10.1038/nature00852

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