FIGURE 2. Single cell origin of mMAPC and rMAPC cultures initiated from ten cells per well, and their differentiated progeny (see also Supplementary Information Table 2).

Mouse or rat BMMNCs, transduced with an MSCV-eGFP retrovirus⁴⁹, were depleted of CD45⁺ TER119⁺ cells, and eGFP⁺ cells were replated at ten cells per well and expanded for more than 100 population doublings. DNA from MAPCs or their differentiated progeny (Fig. 3 and Supplementary Information Fig.3) was digested overnight with EcoRI or BamHI (cut only once in the MSCV-eGFP provirus), fragments were separated by electrophoresis, and probed with a ³²P-labelled eGFP cDNA probe. a, DNA extracted from pooled mMAPCs differentiated after 80 population doublings into endothelial, neuroectodermal and hepatic cells (see Fig. 3). A single retroviral insert at 7 kb was seen. b, Undifferentiated rMAPCs (lane 1) and seven subpopulations of rMAPCs after 75 population doublings, subcultured at 100 cells and expanded for 20 population doublings (lanes 2–8). A single retroviral insert at 6 kb was seen. c, rMAPCs expanded for 85 population doublings differentiated to the endothelial (lane 1), neuroectodermal (lane 2) and hepatic (lane 3) cells. A single retroviral insert at 6 kb was seen.