FIGURE 1. Characteristics of mMAPCs.
From the following article:
Pluripotency of mesenchymal stem cells derived from adult marrow
Yuehua Jiang, Balkrishna N. Jahagirdar, R. Lee Reinhardt, Robert E. Schwartz, C. Dirk Keene, Xilma R. Ortiz-Gonzalez, Morayma Reyes, Todd Lenvik, Troy Lund, Mark Blackstad, Jingbo Du, Sara Aldrich, Aaron Lisberg, Walter C. Low, David A. Largaespada and Catherine M. Verfaillie
Nature 418, 41-49(4 July 2002)
doi:10.1038/nature00870
Mouse BMMNCs were plated with EGF, PDFG-BB, and LIF on FN-coated plates. After 3–4 weeks, bead-selected CD45-/TER119- cells were plated at ten cells per well in FN-coated 96-well plates in the same medium, and expanded at 0.5–1.5 
103 cm-2. a, Cells were enumerated at each passage under a haemocytometer. b, mMAPCs cultured for 120 population doublings were labelled with FITC-coupled antibodies against CD45, Gr-1, Mac-1, CD19, CD3, CD13, c-Kit, SSEA-1, Sca-1, CD34, Thy-1, Flk-1, MHC class II (I–Ak), MHC class I (H–2Kk), CD44 or immunoglobulin isotype control antibodies. Cells were analysed using a FACS-Calibur. Black line, control immunoglobulin; red line, specific antibody. c, Telomere length of mMAPCs cultured for 40 population doublings (PDs) (lane 1) and 102 population doublings (lane 2)28. d, Quantitative RT–PCR for Rex-1 (left), Oct-4 (middle) and 
-actin (right). Lane 1, ladder; lane 2, ES cells; lane 3, MAPCs; lane 4, no-template control (see Methods and Supplementary Information Table 1).
