Figures and Tables

From the following article:

VEGF regulates haematopoietic stem cell survival by an internal autocrine loop mechanism

Hans-Peter Gerber, Ajay K. Malik, Gregg P. Solar, Daniel Sherman, Xiao Huan Liang, Gloria Meng, Kyu Hong, James C. Marsters and Napoleone Ferrara

Nature 417, 954-958(27 June 2002)

doi:10.1038/nature00821

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Figure 1

Real-time RT–PCR analysis of BMMNCs and HSCs and competitive repopulation frequencies of VEGF-deficient HSCs.

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Figure 2

Comparison between the competitive repopulation of CD45.2-positive Sca+ Kit+ Linlo bone marrow cells deleted in both VEGF alleles (VEGFflox/flox) or control (VEGFlox/lox) cells 8 weeks after transplantation into lethally irradiated CD45.1-positive recipient mice.

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Figure 3

VEGF inactivation by Flt–IgG interferes with the paracrine but not autocrine mechanism and small-molecule inhibitors targeting VEGFR-1 and -2 block both functions.

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Table 1

Competitive repopulation assay with HPCs and BMMNCs

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Table 2

Colony formation and repopulation after retroviral transduction

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Table 3

Effect of poly(I:C) treatment on in vitro colony formation

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Table 4

In vitro clonogenic assay

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