FIGURE 2. Inositol reverses the effects of mood-stabilizing drugs on growth cone collapse and spread area.

a, Collapsed growth cones (top left panels) and spread growth cones (right panels) showing stable microtubules (acetylated tubulin) and cytoplasm (calcein).The area of this growth cone was calculated as 122 m² as shown (bottom left panel) Scale bar, 10 m. b, Histograms show the frequency distribution of collapsed growth cones (grey first bar) and the spread area of growth cones plotted in increments of 10 m² (black bars) expressed as a percentage of the total. The drug treatment for left-hand panels is indicated on each plot. The right-hand panels are from cultures treated with drugs in the presence of 1 mM inositol, or cultures treated with TSA alone as indicated. Data were pooled from up to 5 different platings; the total number of growth cones scored for each treatment was about 150.