Supplementary Information for "Crystal structure of the anthrax lethal factor"
Nature, V414, 229

 

Table 1: Data collection summary for Lethal Factor crystals.

Data set

P21 Native

Trimethyl-lead

Hexaiodo- platinate

I4132 MAPKK-2

Resolution range/Å

15-2.2

15-3.3

15-3.5

20-3.9

Reflections: total/unique

523,147/

114,503

198,682/

67,718*

168,592/

26,677

87,382/

23,687

Completeness % (outer shell)

89.5 (88.0)

92.0 (92.0)

83.0 (85.0)

88.1 (86.1)

Rsym % (outer shell)�

5.3 (27.7)

5.3 (13.5)

6.0 (14.0)

10.3 (63.1)

I/sI (outer shell)

16.9 (3.1)

11.3 (5.3)

11.7 (4.8)

12.9 (2.0)

Wavelength

0.89

0.950/0.948/

0.855

1.542

0.97

Phasing Power

 

0.89

1.0 (to 4.0Å)

 

 

�Rsym = S|I - <I>|/S<I>, where I is the observed intensity, <I> is the average intensity from multiple observations of symmetry-related reflections. Phasing power is defined as the root-mean-square (|FH|/E), where |FH| is the RMS heavy-atom structure factor amplitude and E the residual lack of closure error.

*Includes anomalous pairs.

 


Figure 1