FIGURE 3. Differential responsiveness of wild-type and mutant NOD2 to LPS.
From the following article:
A frameshift mutation in NOD2 associated with susceptibility to Crohn's disease
Yasunori Ogura, Denise K. Bonen, Naohiro Inohara, Dan L. Nicolae, Felicia F. Chen, Richard Ramos, Heidi Britton, Thomas Moran, Reda Karaliuskas, Richard H. Duerr, Jean-Paul Achkar, Steven R. Brant, Theodore M. Bayless, Barbara S. Kirschner, Stephen B. Hanauer, Gabriel Nuñez and Judy H. Cho
Nature 411, 603-606(31 May 2001)
doi:10.1038/35079114
a, HEK293T cells were co-transfected in triplicate with the indicated amounts of pcDNA3 (vector), wild-type pcDNA3-NOD2, or pcDNA3-NOD2 3020insC and pEF-BOS-
-gal and pBVI-luc reporter plasmids. Values represent means 
s.d. Expression of wild-type and mutant NOD2 proteins in cell extracts is shown on top. b, HEK293T cells were co-transfected in triplicate with 0.3 ng of pcDNA3-NOD2, 3 ng of pcDNA3-NOD2 3020insC, 3 ng of pcDNA3-TLR4 plus 3 ng of pcDNA3-MD-2 (indicated by TLR4) or pcDNA3 (vector) and pEF-BOS--gal and pBXIV-luc. Under these conditions, both wild-type and mutant NOD2 constructs induced similar levels of basal NF-
B activity. Eight hours after transfection, cells were treated with 10 
g ml-1 of LPS from indicated bacteria. Values represent means s.d. Results are representative of at least five independent experiments.
