Supplementary Information for "Sustainability of three apple production systems"
Nature, v410, 926

Figure 1

Fruit size of apples from three production systems. Differences between values in a year followed by different letters are significant at the 0.05 level (LSD).


Figure 2

Size distribution of apples in 1998 and 1999 from three production systems. The difference between the organic and conventional fruit size distributions resulted in an average 20% reduction in organic fruit value.

 

Figure 3

Trunk cross-sectional area (TCSA) of apple trees grown in three production systems. No differences in growth were detected in any year between treatments at the 0.05 level (LSD).

Figure 4

The study area of four replicate plots for each of the three apple production systems. Each plot contains four rows of approximately 80 trees per row trained on a two-wire trellis system. Trees were planted at a spacing of 1.4 m within rows and 3.2 m between rows for a density of 2240 trees per hectare. The soil on all 12 plots is a coarse-loamy, mixed, mesic Xerifluventic Haplocambid (FAO: Haplic Cambisol). We kept the size of the study area to 1.7 hectares to maintain uniformity of this one soil type. Extending the study area to the west or north would have included different soil types. Permanent pasture areas to the east and south belonged to a neighbor. Prior to installation of the experimental orchard the site had been in grass pasture which was tilled to a depth of 30 cm in January 1994. Soil samples were taken from each of the designated plots following the planting of trees but prior to implementation of management treatments. Analyses of pertinent soil morphological, physical, chemical, and biological properties revealed no significant differences between treatments at that time. Grass corridors (5 m wide) surround the study area and another one cuts through the middle of the study area. These grass corridors act as buffers from the conventional commercial orchards to the north and west and as passageways to beneficials from pastures to the south and east (Thies, C., Tscharntke, T., Landscape structure and biological control in agroecosystems, Science, 285, 893-895, 1999). As an additional buffer, the two treatments (conventional-1 and integrated-4) furthest to the west had an additional fifth row of trees. In these two plots, soil and plant samples were taken in the third and fourth rows from the western edge. With grass corridors, tree-row buffers, and sampling in middle rows only, the efficacy of pest control and fertilization for each treatment was not compromised by plot size. The 20 cm of average annual precipitation at the site is supplemented with an under-tree sprinkler irrigation system.

 

Table A1. Market grade of apples from three production systems. Prior to 1998 all fruit was sold for processing. Differences between values in a year followed by different letters are significant at the 0.05 level (LSD).

Grade

Year

Organic

Conventional

Integrated

Washington Extra Fancy (%)

1998

0.00

0.00

0.00

1999

73.24 a

70.06 a

74.18 a

Washington Fancy (%)

1998

26.62 a

33.65 a

28.97 a

1999

0.00

0.00

0.00

Processed (%)

1998

73.38 a

66.35 a

71.03 a

1999

26.76 a

29.94 a

25.82 a

 

Table A2. Leaf tissue nutrient analyses of three apple production systems. Differences between values in a year followed by different letters are significant at the 0.05 level (LSD).

Nutrient

Year

Organic

Conventional

Integrated

Nitrogen

(%)

1995

2.57 a

2.63 a

2.64 a

1996

2.41 a

2.42 a

2.66 b

1997

2.86 a

2.94 a

2.92 a

1998

2.25 a

2.37 a

2.34 a

1999

2.23 a

2.36 a

2.38 a

Phosphorus

(%)

1995

0.25 a

0.24 a

0.24 a

1996

0.21 a

0.18 b

0.19 ab

1997

0.18 a

0.19 a

0.20 a

1998

0.16 a

0.16 a

0.16 a

1999

0.21 a

0.18 b

0.17 ab

Potassium

(%)

1995

1.95 a

2.03 b

1.95 a

1996

2.23 a

2.00 a

2.08 a

1997

1.25 a

1.38 a

1.58 b

1998

1.40 a

1.73 b

1.75 b

1999

1.83 a

1.85 a

1.90 a

Sulphur

(%)

1995

0.21 a

0.23 a

0.24 a

1996

0.19 a

0.21 a

0.20 a

1997

0.17 a

0.17 a

0.16 a

1998

0.11 a

0.11 a

0.12 a

1999

0.15 a

0.16 a

0.14 a

Calcium

(%)

1995

1.62 a

1.71 a

1.62 a

1996

1.68 a

1.84 b

1.69 a

1997

1.74 a

1.73 a

1.68 a

1998

1.70 a

2.09 a

1.93 a

1999

1.83 a

1.86 a

1.87 a

Magnesium

(%)

1995

0.32 a

0.35 b

0.35 b

1996

0.37 a

0.38 a

0.37 a

1997

0.34 a

0.31 b

0.32 b

1998

0.38 a

0.41 a

0.40 a

1999

0.34 a

0.31 a

0.33 a

Boron

(ppm)

1995

22.75 a

24.50 ab

25.25 b

1996

21.50 a

26.00 b

22.25 a

1997

27.75 a

25.75 a

26.25 a

1998

20.50 a

23.00 a

20.50 a

1999

18.75 a

17.75 a

17.50 a

Zinc

(ppm)

1995

11.75 a

13.25 ab

14.50 b

1996

15.75 a

25.25 b

14.50 a

1997

10.75 a

12.75 a

12.00 a

1998

10.75 a

13.00 b

12.50 b

1999

14.25 a

15.00 a

14.75 a

Manganese

(ppm)

1995

51.00 a

59.25 a

58.75 a

1996

51.75 a

58.75 b

56.25 ab

1997

61.75 a

60.75 a

58.75 a

1998

61.50 a

59.00 a

63.75 a

1999

47.75 a

46.25 a

50.25 a

Copper

(ppm)

1995

9.00 a

8.75 a

8.75 a

1996

9.50 a

10.25 a

9.25 a

1997

8.50 a

8.25 a

8.00 a

1998

8.50 a

8.00 a

8.25 a

1999

8.50 a

8.50 a

9.00 a

Iron

(ppm)

1995

188.00 a

138.75 ab

122.50 b

1996

281.75 a

354.75 a

247.50 a

1997

159.00 a

135.75 ab

116.25 b

1998

190.75 a

172.50 a

188.75 a

1999

315.50 a

259.25 a

296.50 a

 

Table A3. Fruit tissue nutrient analyses of three apple production systems. Fruit was not analyzed for tissue content in 1996. Differences between values in a year followed by different letters are significant at the 0.05 level (LSD).

Nutrient

Year

Organic

Conventional

Integrated

Nitrogen

(%)

1995

0.36 a

0.40 a

0.40 a

1997

0.26 a

0.32 ab

0.35 b

1998

0.40 a

0.47 ab

0.54 b

1999

0.30 a

0.40 b

0.41 b

Phosphorus

(%)

1995

0.135 a

0.130 a

0.140 a

1997

0.070 a

0.088 b

0.075 ab

1998

0.090 a

0.100 a

0.100 a

1999

0.073 a

0.080 b

0.075 ab

Potassium

(%)

1995

0.97 a

0.95 a

1.00 a

1997

0.72 a

0.85 b

0.80 ab

1998

0.83 a

0.90 a

0.85 a

1999

0.80 a

0.88 a

0.88 a

Calcium

(%)

1995

0.04 a

0.03 a

0.03 a

1997

0.04 a

0.04 a

0.05 a

1998

0.06 a

0.07 a

0.06 a

1999

0.10 a

0.10 a

0.09 a

Magnesium

(%)

1995

0.05 a

0.04 b

0.05 ab

1997

0.04 a

0.04 a

0.04 a

1998

0.05 a

0.05 a

0.05 a

1999

0.05 a

0.05 a

0.05 a

Boron

(ppm)

1995

7.75 a

7.75 a

9.75 b

1997

6.75 a

15.50 b

13.75 b

1998

5.00 a

7.00 ab

7.25 b

1999

7.75 a

11.50 b

7.50 a

Zinc

(ppm)

1995

3.75 a

2.00 b

3.00 a

1997

4.25 a

5.00 a

4.50 a

1998

4.50 a

5.25 a

4.50 a

1999

4.25 a

6.75 a

5.00 a

 

Table A4. Fruit maturity analyses of apples from three production systems (a) at harvest, (b) following 3 months controlled atmosphere storage, and (c) following 6 months controlled atmosphere storage. Analyses were not carried out prior to 1998. Differences between values in a year followed by different letters are significant at the 0.05 level (LSD).

Fruit Maturity

Year

Organic

Conventional

Integrated

(a) At Harvest

Firmness

(N)

1998

58.03 a

52.84 b

50.44 b

1999

66.91 a

65.53 ab

64.82 b

Soluble Solids

(Brix)

1998

15.32 a

15.53 a

14.58 b

1999

14.61 a

14.05 b

14.59 a

Titratable acidity

(%)

1998

0.77 a

0.83 ab

0.85 b

1999

0.71 a

0.74 b

0.75 b

Ratio of soluble solids to

titratable acidity

1998

19.81 a

18.81 ab

17.10 b

1999

20.71 a

19.15 b

19.44 b

(b) 3 Months Storage

 

Firmness

(N)

1998

57.11 a

56.15 a

56.09 a

1999

58.00 a

57.01 b

57.34 ab

Soluble Solids

(Brix)

1998

14.77 a

14.42 a

13.77 a

1999

15.59 a

14.96 b

15.24 ab

Titratable acidity

(%)

1998

0.63 a

0.66 ab

0.71 b

1999

0.59 a

0.62 b

0.61 b

Ratio of soluble solids to

titratable acidity

1998

23.53 a

21.88 a

19.46 b

1999

26.81 a

24.19 b

24.87 b

(c) 6 Months Storage

   

Firmness

(N)

1998

56.22 a

57.84 a

58.24 a

1999

56.37 a

53.40 b

54.66 ab

Soluble Solids

(Brix)

1998

14.27 a

15.01 a

14.34 a

1999

15.61 a

14.90 b

15.56 a

Titratable acidity

(%)

1998

0.56 a

0.61 ab

0.64 b

1999

0.42 a

0.43 ab

0.44 b

Ratio of soluble solids to

titratable acidity

1998

25.87 a

24.88 ab

22.56 b

1999

36.90 a

34.55 b

35.21 b

 

 

Table A5. Consumer taste preferences of apples from three production systems (a) at harvest and (b) following 6 months controlled atmosphere storage. Preference tests were conducted only in 1999. Differences between values in a test category followed by different letters are significant at the 0.05 level (LSD).

Taste Parameter

Organic

Conventional

Integrated

(a) At Harvest

Overall Acceptance

(1 = Dislike extremely;

9 = Like extremely)

6.3 a

6.8 a

6.2 a

Texture

(1 = Dislike extremely;

9 = Like extremely)

6.6a

7.1 a

6.7 a

Flavor

(1 = Dislike extremely;

9 = Like extremely)

6.3 a

6.6 a

6.1 a

Firmness

(1 = Very Soft;

9 = Very hard)

6.3 a

6.3 a

6.1 a

Sweetness

(1 = Not at all sweet;

9 = Extremely sweet)

5.5 a

5.3 a

5.0 a

Tartness

(1 = Not at all tart;

9 = Extremely tart)

4.6 a

5.6 b

5.5 b

(b) 6 Months Storage

Overall Acceptance

(1 = Dislike extremely;

9 = Like extremely)

6.1 a

6.0 a

6.5 a

Texture

(1 = Dislike extremely;

9 = Like extremely)

6.1 a

5.9 a

5.9 a

Flavor

(1 = Dislike extremely;

9 = Like extremely)

6.0 a

5.9 a

6.7 b

Firmness

(1 = Very Soft;

9 = Very hard)

5.5 a

5.3 a

5.1 a

Sweetness

(1 = Not at all sweet;

9 = Extremely sweet)

5.6 a

5.0 b

5.6 a

Tartness

(1 = Not at all tart;

9 = Extremely tart)

3.6 a

4.7 b

4.8 b

 

Table A6. Cumulative environmental impact ratings of four apple production systems from 1994 to 1999. Total points = A X B X C. In some cases, the total points may appear not to equal the product of A X B X C due to rounding errors.

<
     

(A)

(B)

(C)

 

Treatment

Product

name

Chemical

name

No. of

applications

Rate

(amount

application-1

ha-1)

Points per unit amount

Total points

Organic

Dipel

Bt

5

2.2

kg

7.28

81.5

 

Isomate C

Pheromone

4

988.0

ties

0.02

59.3

 

Microthiol

Sulfur

11

11.2

kg

2.32

285.3

 

Superior Oil

Oil

5

18.7

liters

0.42

39.5

Total

465.6

Conventional

Captan

Captan

1

14.0

liters

4.02

56.3

Dipel

Bt

4

2.2

kg

7.28

65.2

Ethrel

Ethephon

7

2.1

liters

2.33

34.2

Guthion

Azinphos methyl

16

2.2

kg

17.64

632.3

 

Isomate C

Pheromone

4

988.0

ties

0.015

59.2

 

Lorsban

Chlorpyrifos

7

1.2

liters

10.57

86.5

 

Microthiol

Sulfur

9

11.2

kg

2.35

244.5

 

Provado

Imidacloprid

3

0.1

liters

274.84

57.8

 

Procure

Triflumizole

4

0.6

kg

18.70

41.9

 

Rally

Myclobutanil

7

0.4

liters

131.92

323.7

 

Roundup

Glyphosate

23

4.7

liters

9.51

1022.6

 

Rubigan

Fenarimol

1

0.4

liters

83.52

33.4

 

Simazine

Simazine

3

2.3

liters

8.25

57.8

 

Sevin

Carbaryl

2

1.8

liters

13.53

47.4

 

Solicam

Norflurazon

3

2.2

kg

10.81

90.8

 

Superior Oil

Oil

5

18.7

liters

0.42

39.5

 

Total

         

2,893.2

Integrated

Captan

Captan

1

14.0

liters

4.02

56.3

 

Dipel

Bt

4

2.2

kg

7.28

65.2

 

Ethrel

Ethephon

7

2.1

liters

2.33

34.2

 

Guthion

Azinphos methyl

16

2.2

kg

17.64

632.3

 

Isomate C

Pheromone

4

988.0

ties

0.015

59.2

 

Lorsban

Chlorpyrifos

7

1.2

liters

10.57

86.5

 

Microthiol

Sulfur

9

11.2

kg

2.32

244.5

 

Provado

Imidacloprid

3

0.1

liters

274.84

57.8

 

Procure

Triflumizole

4

0.6

kg

18.70

41.9

 

Rally

Myclobutanil

7

0.4

liters

131.92

323.7

 

Roundup

Glyphosate

11

4.7

liters

9.30

480.8

 

Rubigan

Fenarimol

1

0.4

liters

83.52

33.4

 

Sevin

Carbaryl

2

1.8

liters

13.53

47.4

 

Superior Oil

Oil

5

18.7

liters

0.42

39.5

 

Total

         

2,211.1

Non-PMD Conventional

Guthion

Azinphos Methyl

14

2.2

kg

17.64

553.3

 

Dipel

Bt

8

2.2

kg

7.28

130.4

 

Carbaryl

Carbaryl

6

2.1

liters

13.5

170.7

 

Lorsban

Chlorpyrifos

4

4.7

liters

10.6

197.6

 

Ethrel

Ethephon

3

2.1

liters

2.33

14.7

 

Rubigan

Fenarimol

6

0.3

liters

114.38

200.4

 

Roundup

Glyphosate

8

4.7

liters

9.51

355.7

 

Provado

Imidacloprid

5

0.1

liters

274.8

96.3

 

Lime Sulfur

Lime Sulfur

11

37.4

liters

2.11

869.4

 

Supracide

Methidathion

1

0.6

liters

9.85

5.8

 

Rally

Myclobutanil

5

0.4

liters

131.9

231.2

 

NAA 200

NAA

3

0.2

liters

76.1

34.7