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Letters to Nature

Nature 409, 1047-1051 (22 February 2001) | doi:10.1038/35059098; Received 4 September 2000; Accepted 22 December 2000

The voltage-sensitive sodium channel is a bell-shaped molecule with several cavities

Chikara Sato1, Yutaka Ueno1, Kiyoshi Asai1, Katsutoshi Takahashi2, Masahiko Sato3, Andreas Engel4 & Yoshinori Fujiyoshi5

  1. Supermolecular Science Division, Electrotechnical Laboratory (ETL), Umezono 1-1-4, Tsukuba, 305-8568 Japan
  2. School of Knowledge Science, Japan Advanced Institute of Science and Technology Hokuriku (JAIST), Asahidai 1-1, Tatsunokuchi, Ishikawa, 923-1211, Japan
  3. Central Research Institute, Itoham Foods Inc., Kubogaoka 1-2, Moriya, 302-0104, Japan
  4. Maurice E. Müller Institute, at the Biozentrum, University of Basel, Klingelbergstrasse 70, CH-4056 Basel, Switzerland
  5. Department of Biophysics, Faculty of Science, Kyoto University, Oiwake, Kitashirakawa, Sakyo-ku, Kyoto, 606-8502, Japan

Correspondence to: Chikara Sato1 Correspondence and requests for materials should be addressed to C.S. (e-mail: Email: tisato@etl.go.jp).

Top

Voltage-sensitive membrane channels, the sodium channel, the potassium channel and the calcium channel operate together to amplify, transmit and generate electric pulses in higher forms of life. Sodium and calcium channels are involved in cell excitation, neuronal transmission, muscle contraction and many functions that relate directly to human diseases1, 2, 3, 4. Sodium channels—glycosylated proteins with a relative molecular mass of about 300,000 (ref. 5)—are responsible for signal transduction and amplification, and are chief targets of anaesthetic drugs6 and neurotoxins1. Here we present the three-dimensional structure of the voltage-sensitive sodium channel from the eel Electrophorus electricus. The 19 Å structure was determined by helium-cooled cryo-electron microscopy and single-particle image analysis of the solubilized sodium channel. The channel has a bell-shaped outer surface of 135 Å in height and 100 Å in side length at the square-shaped bottom, and a spherical top with a diameter of 65 Å. Several inner cavities are connected to four small holes and eight orifices close to the extracellular and cytoplasmic membrane surfaces. Homologous voltage-sensitive calcium and tetrameric potassium channels, which regulate secretory processes and the membrane potential7, may possess a related structure.