FIGURE 2. Idealized representation of the hierarchical shotgun sequencing strategy.
From the following article:
Initial sequencing and analysis of the human genome
and International Human Genome Sequencing Consortium
Nature 409, 860-921(15 February 2001)
A library is constructed by fragmenting the target genome and cloning it into a large-fragment cloning vector; here, BAC vectors are shown. The genomic DNA fragments represented in the library are then organized into a physical map and individual BAC clones are selected and sequenced by the random shotgun strategy. Finally, the clone sequences are assembled to reconstruct the sequence of the genome.