Human Genome
Nature 409, 947-948 (15 February 2001) | doi:10.1038/35057177; Received 20 October 2000; Accepted 21 December 2000
A physical map of human chromosome 14
Thomas Brüls1, Gabor Gyapay1, Jean-Louis Petit1, François Artiguenave1, Virginie Vico1, Shizen Qin2, Aye Mon Tin-Wollam3, Corinne Da Silva1, Delphine Muselet1, Delphine Mavel1, Eric Pelletier1, Michael Levy1, Asao Fujiyama4, Fumihiko Matsuda5, Richard Wilson3, Lee Rowen2, Leroy Hood2, Jean Weissenbach1, William Saurin1 & Roland Heilig1
We report the construction of a tiling path of around 650 clones covering more than 99% of human chromosome 14. Clone overlap information to assemble the map was derived by comparing fully sequenced clones with a database of clone end sequences1, 2 (sequence tag connector strategy). We selected homogeneously distributed seed points using an auxiliary high-resolution radiation hybrid map comprising 1,895 distinct positions. The high long-range continuity and low redundancy of the tiling path indicates that the sequence tag connector approach compares favourably with alternative mapping strategies.
- Genoscope and CNRS UMR 8030, 2 Rue Gaston Cremieux, CP 5706, 91057 Evry Cedex, France
- Multimegabase Sequencing Center, The Institute for Systems Biology, 4225 Roosvelt Way, NE Suite 200, Seattle, Washington 98105, USA
- Washington University Genome Sequencing Center, Box 8501, 4444 Forest Park Avenue, St. Louis, Missouri 63108, USA
- RIKEN Genome Sciences Center, RIKEN, 1-7-22, Tsurumi-ku, Yokohama-city, Kanagawa 230-0045, Japan
- Centre National de Genotypage, 2 Rue Gaston Cremieux, CP 5721, 91057 Evry Cedex, France
Correspondence to: Roland Heilig1 Correspondence and requests for materials should be addressed to R.H. (e-mail: heilig@genoscope.cns.fr).
