Human Genome
Nature 409, 942-943 (15 February 2001) | doi:10.1038/35057165; Received 28 November 2000; Accepted 21 December 2000
The physical maps for sequencing human chromosomes 1, 6, 9, 10, 13, 20 and X
D. R. Bentley1, P. Deloukas1, A. Dunham1, L. French1, S. G. Gregory1, S. J. Humphray1, A. J. Mungall1, M. T. Ross1, N. P. Carter1, I. Dunham1, C. E. Scott1, K. J. Ashcroft1, A. L. Atkinson1, K. Aubin1, D. M. Beare1, G. Bethel1, N. Brady1, J. C. Brook1, D. C. Burford1, W. D. Burrill1, C. Burrows1, A. P. Butler1, C. Carder1, J. J. Catanese2, C. M. Clee1, S. M. Clegg1, V. Cobley1, A. J. Coffey1, C. G. Cole1, J. E. Collins1, J. S. Conquer1, R. A. Cooper1, K. M. Culley1, E. Dawson1, F. L. Dearden1, R. M. Durbin1, P. J. de Jong2, P. D. Dhami1, M. E. Earthrowl1, C. A. Edwards1, R. S. Evans1, C. J. Gillson1, J. Ghori1, L. Green1, R. Gwilliam1, K. S. Halls1, S. Hammond1, G. L. Harper1, R. W. Heathcott1, J. L. Holden1, E. Holloway1, B. L. Hopkins1, P. J. Howard1, G. R. Howell1, E. J. Huckle1, J. Hughes1, P. J. Hunt1, S. E. Hunt1, M. Izmajlowicz1, C. A. Jones1, S. S. Joseph1, G. Laird1, C. F. Langford1, M. H. Lehvaslaiho1, M. A. Leversha1, O. T. McCann1, L. M. McDonald1, J. McDowall1, G. L. Maslen1, D. Mistry1, N. K. Moschonas3, V. Neocleous4, D. M. Pearson1, K. J. Phillips1, K. M. Porter1, S. R. Prathalingam1, Y. H. Ramsey1, S. A. Ranby1, C. M. Rice1, J. Rogers1, L. J. Rogers1, T. Sarafidou3, D. J. Scott1, G. J. Sharp1, C. J. Shaw-Smith1, L. J. Smink1, C. Soderlund1, E. C. Sotheran1, H. E. Steingruber1, J. E. Sulston1, A. Taylor1, R. G. Taylor1, A. A. Thorpe1, E. Tinsley1, G. L. Warry1, A. Whittaker1, P. Whittaker1, S. H. Williams1, T. E. Wilmer1, R. Wooster1 & C. L. Wright1
We constructed maps for eight chromosomes (1, 6, 9, 10, 13, 20, X and (previously) 22), representing one-third of the genome, by building landmark maps, isolating bacterial clones and assembling contigs. By this approach, we could establish the long-range organization of the maps early in the project, and all contig extension, gap closure and problem-solving was simplified by containment within local regions. The maps currently represent more than 94% of the euchromatic (gene-containing) regions of these chromosomes in 176 contigs, and contain 96% of the chromosome-specific markers in the human gene map. By measuring the remaining gaps, we can assess chromosome length and coverage in sequenced clones.
- The Sanger Centre, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, UK
- Department of Biology, University of Crete and Institute of Molecular Biology and Biotechnology, PO Box 2208, 71409 Heraklion, Crete, Greece
- Neurogenetic Laboratory, The Cyprus Institute of Neurology and Genetics, 6, International Airport Avenue, PO Box 23462, 1683 Nicosia, Cyprus
- Children's Hospital-BACPAC Resources, 747 52nd Street, Oakland, California 94609, USA
Correspondence to: D. R. Bentley1 Correspondence and requests for material should be addressed to D.R.B (e.mail: Email: drb@sanger.ac.uk).
