Processive translocation and DNA unwinding by individual RecBCD enzyme
molecules

From the following article:

Piero R. Bianco, Laurence R. Brewer, Michele Corzett, Rod Balhorn, Yin Yeh, Stephen C. Kowalczykowski and Ronald J. Baskin

Nature 409, 374-378(18 January 2001)

doi:10.1038/35053131

This movie shows unwinding of a single molecule of lambda DNA by a simgle RecBCD enzyme at 37°C in the presence of 1mM ATP.

This movie shows unwinding of a single molecule of lambda DNA by a simgle RecBCD enzyme at 23°C in the presence of 1mM ATP.

This movie shows unwinding of a single molecule of lambda DNA by a simgle RecBCD enzyme at 23°C in the presence of 250M ATP.

This movie shows unwinding of a single molecule of lambda DNA in the absence of cofactor at 23°C.

A graph showing the amount of active protein in the RecBCD enzyme prepatation used in the experiments described.