FIGURE 5. Misplaced FDC-containing germinal centres in BLC-/-
mice and BLC-independent expression of LT
1
2 on germinal centre
B cells.
From the following article:
A chemokine-driven positive feedback loop organizes lymphoid follicles
K. Mark Ansel, Vu N. Ngo, Paul L. Hyman, Sanjiv A. Luther, Reinhold Förster, Jonathon D. Sedgwick, Jeffrey L. Browning, Martin Lipp and Jason G. Cyster
Nature 406, 309-314(20 July 2000)
doi:10.1038/35018581
a, Spleen and lymph nodes from immunized mice stained to detect
non-germinal-centre B cells (IgD) and FDCs (CR1). Staining of serial sections
with peanut agglutinin (PNA), established that the strongly CR1+
areas in the BLC-/- spleen and lymph node were germinal centres
(not shown). Data are representative of eight immunized BLC+/-
and BLC-/- mice. b, LT
1
2 expression on
germinal centre B cells. Histograms show LTR-Ig staining on gated B220
+GL7- non-germinal-centre and B220+GL7
+ germinal centre B cells from mesenteric lymph nodes of immunized
BLC+/- and BLC-/- mice. c, In vitro
CD40 stimulation induces LT12 expression in a CXCR5-independent
manner. Spleen cells were incubated with buffer alone (control) or anti-CD40
antibody for 6 h and then stained. Histograms show LTR-Ig staining
on B220+ cells.
