FIGURE 4. BLC induces LT
1
2 upregulation on B cells in vitro.
From the following article:
A chemokine-driven positive feedback loop organizes lymphoid follicles
K. Mark Ansel, Vu N. Ngo, Paul L. Hyman, Sanjiv A. Luther, Reinhold Förster, Jonathon D. Sedgwick, Jeffrey L. Browning, Martin Lipp and Jason G. Cyster
Nature 406, 309-314(20 July 2000)
doi:10.1038/35018581
a–d, Histograms show LT
R-Ig staining of B220-gated
B cells from in vitro cultures in the absence (Con) or presence of
chemokines at the indicated amounts for 6 h. Cells were pre-incubated
with 200 ng ml-1 PTX or an equal volume of buffer
for 2 h and then incubated with BLC (3 
g ml
-1) for a further 6 h (b), incubated in SLC, ELC or
BLC at 3 g ml-1 or SDF1 at 1 g ml
-1 (c), or incubated with BLC (3 g ml
-1) alone or combined with actinomycin D (ActD, 5 g ml
-1), cycloheximide (CHX, 1 g ml-1)
or wortmannin (WMN, 100 nM) for 6 h (d).
