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Transport, docking and exocytosis of single secretory granules in live chromaffin cells

Nature volume 388, pages 474–478 (1997)Cite this article

Abstract

Neurons maintain a limited pool of synaptic vesicles which are docked at active zones and are awaiting exocytosis1,2,3,4. By contrast, endocrine cells releasing large, dense-core secretory granules have no active zones, and there is disagreement about the size5 and even the existence6 of the docked pool. It is not known how, and how rapidly, secretory vesicles are replaced at exocytic sites in either neurons or endocrine cells. By using electron microscopy, we have now been able to identify a pool of docked granules in chromaffin cells that is selectively depleted when cells secrete. With evanescent-wave fluorescence microscopy7, we observed single granules undergoing exocytosis and leaving behind patches of bare plasmalemma. Fresh granules travelled to the plasmalemma at a top speed of 114 nm s−1, taking an average of 6 min to arrive. On arrival, their motility diminished 4-fold, probably as a result of docking. Some granules detached and returned to the cytosol. We conclude that a large pool of docked granules turns over slowly, that granules move actively to their docking sites, that docking is reversible, and that the ‘rapidly releasable pool’ measured electrophysiologically represents a small subset of docked granules.

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Figure 1: a, Unfixed, quickly frozen chromaffin cell showing plasmalemma (left) and the granules beneath.
Figure 2: a, 1–4, Successive frames at 0.5s−1 show a granule (1, 2) vanishing abruptly owing to exocytosis (2, 3); the granule and its last location are indicated by circles.
Figure 3: a, Images before (1), immediately after (2) and 20 min after the end of a 2-min stimulation with elevated [K+] (3).
Figure 4: a, A granule appearing beneath the plasmalemma.

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Acknowledgements

We thank J. Howard, M. Lindau, W. M. Roberts and T. Soldati for their helpful suggestions on the manuscript.

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  1. Max-Planck-Institut für medizinische Forschung, Jahnstrasse 29, Heidelberg, 69120, Germany

    J. A. Steyer, H. Horstmann & W. Almers

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Correspondence to W. Almers.

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Steyer, J., Horstmann, H. & Almers, W. Transport, docking and exocytosis of single secretory granules in live chromaffin cells. Nature 388, 474–478 (1997). https://doi.org/10.1038/41329

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