Nature 386, 366 - 373 (27 March 1997); doi:10.1038/386366a0

Expression of Radical fringe in limb-bud ectoderm regulates apical ectodermal ridge formation

Ed Laufer^*, Randall Dahn^†, Olivia E. Orozco^*, Chang-Yeol Yeo^*, Jacqueline Pisenti^‡, Domingos Henrique^§, Ursula K. Abbott^‡, John F. Fallon^† & Cliff Tabin^*

^* Department of Genetics, Harvard Medical School, 200 Longwood Avenue, Boston, Massachusetts 02115, USA
^† Department of Anatomy, University of Wisconsin, 1300 University Avenue, Madison, Wisconsin 53706, USA
^‡ Department of Avian Sciences, University of California, Davis, Davis, California 95616, USA
^§ Developmental Genetics Laboratory, Imperial Cancer Research Fund, PO Box 123, 44 Lincoln's Inn Fields, London WC2A 3PX, UK

The apical ectodermal ridge of the vertebrate limb bud lies at the junction of the dorsal and ventral ectoderm and directs patterning of the growing limb. Its formation is directed by the boundary between cells that do and cells that do not express the gene Radical fringe. This is similar to the establishment of the margin cells at the Drosophila wing dorsoventral border by fringe. Radical fringe expression in chick-limb dorsal ectoderm is established in part through repression by Engrailed-1 in the ventral ectoderm.

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