Nature Publishing Group, publisher of Nature, and other science journals and reference works
Nature
my account e-alerts subscribe register
   
Saturday 23 September 2017
Journal Home
Current Issue
AOP
Archive
Download PDF
References
Export citation
Export references
Send to a friend
More articles like this

Letters to Nature
Nature 384, 266 - 270 (21 November 1996); doi:10.1038/384266a0

Muscle progenitor cells failing to respond to positional cues adopt non-myogenic fates in myf-5 null mice

S. Tajbakhsh, D. Rocancourt & M. Buckingham

Unité de Génétique Moléculaire du Développement, CNRS URA1947, Départment de Biologie Moléculaire, Institut Pasteur, 25 rue du Dr Roux, 75724 Paris, Cedex 15, France

MICE that have mutations in both myogenic transcription factors Myf-5 and MyoD totally lack skeletal muscle fibres and their precursor myoblasts1, whereas with either mutation alone, muscle is present2,3. Skeletal muscle in the vertebrate body is derived from epithelial somites that respond to environmental signals to form the dorsal epithelial dermomyotome (dermis, muscle) and ventral mesenchymal sclerotome (axial skeleton, ribs) 4,5. The first muscle, the myotome, forms centrally in the somite, when only myf-5 is programming myogenesis. By targeting the nlacZ reporter gene into the myf-5 locus, we demonstrate that β-galactosidase muscle progenitor cells are present in the dermomyotome of myf-5 null embryos, and that they undergo a normal epithelial-mesenchymal transition; however, they migrate aberrantly. Dorsally, they accumulate under the ectoderm and express a non-muscle dermal marker, Dermo-1. Ventrally, β-galactosidase+ cells also fail to localize correctly, express a cartilage marker scleraxis, and are subsequently found in ribs. Therefore Myf-5 protein is necessary for cells to respond correctly to positional cues in the embryo and to adopt their myogenic fate. In its absence, muscle progenitors, having activated myf-5, remain multipotent and differentiate into other somitic derivatives according to their local environment.

  1. Rudnicki, M. A. et al. Cell 75, 1351−1359 (1993). | Article | PubMed | ISI | ChemPort |
  2. Braun, T., Rudnicki, M. A., Arnold, H.-H. & Jaenisch, R. Cell 71, 369−382 (1992). | Article | PubMed | ISI | ChemPort |
  3. Rudnicki, M. A., Braun, T., Hinuma, S. & Jaenisch, R. Cell 71, 383−390 (1992). | Article | PubMed | ISI | ChemPort |
  4. Cossu, G., Tajbakhsh, S. & Buckingham, M. Trends Genet. 12, 218−223 (1996). | Article | PubMed | ISI | ChemPort |
  5. Christ, B. & Ordahl, C. P. Anatomy Embryol. 191, 381−396 (1995). | ChemPort |
  6. Ott, M.-O., Bober, E., Lyons, G., Arnold, H. & Buckingham, M. Development 111, 1097−1107 (1991). | PubMed | ISI | ChemPort |
  7. Buckingham, M. Trends Genet. 8, 144−149 (1992). | Article | PubMed | ISI | ChemPort |
  8. Tosney, K. W., Dehnbostel, D. B. & Erickson, C. A. Dev. Biol. 163, 389−406 (1994). | Article | PubMed | ChemPort |
  9. Li, L., Cserjesi, P. & Olson, E. N. Dev. Biol. 172, 280−292 (1995). | Article | PubMed | ISI | ChemPort |
  10. Cserjesi, P. et al. Development 121, 1099−1110 (1995). | PubMed | ISI | ChemPort |
  11. Grass, S., Arnold, H. H. & Braun, T. Development 122, 141−150 (1996). | PubMed | ChemPort |
  12. Tajbakhsh, S. & Buckingham, M. E. Proc. Natl Acad. Sci. USA 91, 747−751 (1994). | PubMed | ChemPort |
  13. Siegfried, E. & Perrimon, N. Bioessays 16, 395−404 (1994). | Article | PubMed | ISI | ChemPort |
  14. Epstein, J. A., Shapiro, D. N., Cheng, J., Lam, P. Y. P. & Maas, R. L. Proc. Natl Acad. Sci. USA 93, 4213−4218 (1996). | Article | PubMed | ChemPort |
  15. Yang, X.-M., Vogan, K., Gros, P. & Park, M. Development 122, 2163−2171 (1996). | PubMed | ChemPort |
  16. Hay, E. D. Curr. Opin. Cell Biol. 5, 1029−1035 (1993). | Article | PubMed | ChemPort |
  17. Gurdon, J. B. Nature 336, 772−774 (1988). | Article | PubMed | ISI | ChemPort |
  18. Cossu, G., Kelly, R., Di Donna, S., Vivarelli, E. & Buckingham, M. Proc. Natl Acad. Sci. USA 92, 2254−2258 (1995). | PubMed | ChemPort |
  19. Godsave, S. F. & Slack, J. M. Development 111, 523−530 (1991). | PubMed | ChemPort |
  20. Rong, P. M., Teillet, M. A., Ziller, C. & Le Douarin, N. M. Development 115, 657−672 (1992). | PubMed | ISI | ChemPort |
  21. Bober, E. et al. Development 120, 3073−3082 (1994). | PubMed | ChemPort |
  22. Pownall, M. E., Strunk, K. E. & Emerson, C. P. Jr Development 122, 1475−1488 (1996). | PubMed | ChemPort |
  23. Tajbakhsh, S. et al. Dev. Dyn. 206, 291−300 (1996). | Article | PubMed | ISI | ChemPort |
  24. Magin, T. M., McWhir, J. & Melton, D. W. Nucleic Acids Res. 20, 3795−3796 (1992). | PubMed | ISI | ChemPort |
  25. Tajbakhsh, S. & Buckingham, M. E. Development 121, 4077−4083 (1995). | PubMed | ISI | ChemPort |
  26. Tajbakhsh, S. & Houzelstein, D. Trends Genet. 11, 42 (1995). | Article | PubMed | ChemPort |
  27. Yagi, T. et al. Proc. Natl Acad. Sci. USA 87, 9918−9922 (1990). | PubMed | ChemPort |



© 1996 Nature Publishing Group
Privacy Policy