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Transcriptional regulator of programmed cell death encoded by Caenorhabditis elegans gene ces-2

Abstract

THE ces (for cell-death specification) genes of the nematode Caenorhabditis elegans control the cell-death fate of individual cell types and are candidates for being the regulators of an evolutionarily conserved general pathway of programmed cell death1–4. Here we present what we believe is the first molecular characterization of a ces gene. We cloned the gene ces-2, which is required to activate programmed cell death in the sister cells of the serotoninergic neurosecretory motor (NSM) neurons, and found that ces-2 encodes a basic region leucine-zipper (bZIP) transcription factor. The CES-2 protein is most similar to members of the PAR (proline- and acid-rich) subfamily of bZIP proteins and has DNA-binding specificity like that of PAR-family proteins. An oncogenic form of the mammalian PAR-family protein, hepatic leukaemia factor (HLF), is reported to effect programmed cell death in mammalian cells5. On the basis of these observations, we suggest that some CES-2/PAR family transcription factors are evolutionarily conserved regulators of programmed cell death.

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References

  1. Ellis, R. E. & Horvitz, H. R. Development 112, 591–603 (1991).

    CAS  PubMed  Google Scholar 

  2. Horvitz, H. R., Shaham, S. & Hengartner, M. O. Cold Spring Harbor Symp. quant. Biol. 59, 377–385 (1994).

    Article  CAS  Google Scholar 

  3. Wyllie, A. H. Curr. Opin. Genet. Dev. 5, 97–104 (1995).

    Article  CAS  Google Scholar 

  4. Hengartner, M. O. Curr. Opin. Genet. Dev. 6, 34–38 (1996).

    Article  CAS  Google Scholar 

  5. Inaba, T. et al. Nature 382, 541–544 (1996).

    Article  ADS  CAS  Google Scholar 

  6. Krause, M. & Hirsh, D. Cell 49, 753–761 (1987).

    Article  CAS  Google Scholar 

  7. Sulston, J. E., Schierenberg, E., White, J. G. & Thomson, J. N. Devl. Biol. 100, 64–119 (1983).

    Article  CAS  Google Scholar 

  8. Hurst, H. C. Transcription Factors 1: bZIP Proteins. (Academic, London, 1994).

    Google Scholar 

  9. Hunger, S. P., Ohyashihiki, K., Toyama, K. & Cleary, M. L. Genes Dev. 6, 1608–1620 (1992).

    Article  CAS  Google Scholar 

  10. Inaba, T. et al. Science 257, 531–534 (1992).

    Article  ADS  CAS  Google Scholar 

  11. Mueller, C. R., Maire, P. & Schibler, U. Cell 61, 279–291 (1990).

    Article  CAS  Google Scholar 

  12. Drolet, D. W. et al. Genes Dev. 5, 1739–1753 (1991).

    Article  CAS  Google Scholar 

  13. Iyer, S. V., Davis, D. L., Seal, S. N. & Burch, J. B. E. Molec. cell. Biol. 11, 4863–4875 (1991).

    Article  CAS  Google Scholar 

  14. Cowell, I. G., Skinner, A. & Hurst, H. C. Molec. cell. Biol. 12, 3070–3077 (1992).

    Article  CAS  Google Scholar 

  15. Zhang, W. et al. Molec. cell. Biol. 15, 6055–6063 (1995).

    Article  CAS  Google Scholar 

  16. Capovilla, M., Eldon, E. D. & Pirrotta, V. Development 114, 99–112 (1992).

    CAS  PubMed  Google Scholar 

  17. Inaba, T. et al. Molec. cell. Biol. 14, 3403–3413 (1994).

    Article  CAS  Google Scholar 

  18. Hunger, S. P., Brown, R. & Cleary, M. L. Molec. cell. Biol. 14, 5986–5996 (1994).

    Article  CAS  Google Scholar 

  19. Haas, N. B., Cantwell, C. A., Johnson, P. F. & Burch, J. B. Molec. cell. Biol. 15, 1923–1932 (1995).

    Article  CAS  Google Scholar 

  20. Ellenberger, T. E., Brandl, C. J., Struhl, K. & Harrison, S. C. Cell 71, 1223–1237 (1992).

    Article  CAS  Google Scholar 

  21. Glover, J. N. & Harrison, S. C. Nature 373, 257–261 (1995).

    Article  ADS  CAS  Google Scholar 

  22. Pu, W. T. & Struhl, K. Molec. cell. Biol. 11, 4918–4926 (1991).

    Article  CAS  Google Scholar 

  23. Small, S., Kraut, R., Hoey, T., Warrior, R. & Levine, M. Genes Dev. 5, 827–839 (1991).

    Article  CAS  Google Scholar 

  24. Yoshihara, T., Inaba, T., Shapiro, L. H., Kato, J. Y. & Look, A. T. Molec. cell. Biol. 15, 3247–3255 (1995).

    Article  CAS  Google Scholar 

  25. Coulson, A. et al. BioEssays 13, 413–417 (1991).

    Article  CAS  Google Scholar 

  26. Sambrook, J., Fritsch, E. F. & Maniatis, T. Molecular Cloning: A Laboratory Manual (Cold Spring Harbor Laboratory Press, NY 1989).

    Google Scholar 

  27. Mello, C. C., Kramer, J. M., Stinchcomb, D. T. & Ambros, V. EMBO J. 10, 3959–3970 (1991).

    Article  CAS  Google Scholar 

  28. Barstead, R. J. & Waterston, R. H. J. biol. Chem. 264, 10177–10185 (1989).

    CAS  Google Scholar 

  29. Okkema, P. G. & Fire, A. Development 120, 2175–2186 (1994).

    CAS  PubMed  Google Scholar 

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Metzstein, M., Hengartner, M., Tsung, N. et al. Transcriptional regulator of programmed cell death encoded by Caenorhabditis elegans gene ces-2. Nature 382, 545–547 (1996). https://doi.org/10.1038/382545a0

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