Abstract
IN many animal groups, factors required for germline formation are localized in germ plasm1, a region of the egg cytoplasm. In Drosophila embryos, germ plasm is located in the posterior pole region and is inherited in pole cells, the germline progenitors. Transplantation experiments have demonstrated that germ plasm contains factors that can form germline2–4, and germ plasm also directs abdomen formation5. Genetic analysis has shown that a common mechanism directs the localization of the abdomen and germline-forming factors to the posterior pole6–12. The critical factor for abdomen formation is the nanos (nos) protein (nanos)13–15. Here we show that nos is also essential for germline formation in Drosophila; pole cells lacking nanos activity fail to migrate into the gonads, and so do not become functional germ cells. In such pole cells, gene expression, which normally initiates within the gonad, begins prematurely during pole-cell migration. Premature activation of genes in germline precursors may mean that these cells fail to develop normally. A function for nos protein in Drosophila germline formation is compatible with observations of its association with germ plasm in other animals16–18.
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References
Eddy, E. M. Int. Rev. Cytol. 43, 229–280 (1975).
Illmensee, K. & Mahowald, A. P. Proc. natn. Acad. Sci. U.S.A. 71, 1016–1020 (1974).
Illmensee, K. & Mahowald, A. P. Exp. Cell Res. 97, 127–140 (1976).
Okada, M. et al. Devl Biol. 37, 43–54 (1974).
Frohnhöfer, H. G. et al. J. Embryol. exp. Morph. 97, 169–179 (1986).
Lehmann, R. & Nüsslein-Volhard, C. Cell 47, 141–152 (1986).
Manseau, L. J. & Schüpbach, T. Genes Dev. 3, 1437–1452 (1989).
Schüpbach, T. & Wieschaus, E. Wilhelm Roux Arch. dev. Biol. 195, 302–317 (1986).
Boswell, R. E. & Mahowaid, A. P. Cell 43, 97–104 (1985).
Boswell, R. E. et al. Development 113, 373–384 (1991).
Ephrussi, A. & Lehmann, R. Nature 358, 387–392 (1992).
Smith, J. L. et al. Cell 70, 849–859 (1992).
Lehmann, R. & Nüsslein-Volhard, C. Development 112, 679–691 (1991).
Wang, C. & Lehmann, R. Cell 66, 637–647 (1991).
Wang, C. et al. Dev. Dynam. 199, 103–115 (1994).
Curtis, D. et al. Development 121, 1899–1910 (1995).
Forristall, C. et al. Development 121, 201–208 (1995).
Mosquera, L. et al. Development 117, 377–386 (1993).
Barker, D. D. et al. Genes Dev. 6, 2312–2326 (1992).
Hülskamp, M. et al. Nature 338, 629–632 (1989).
Irish, V. et al. Nature 338, 646–648 (1989).
Struhl, G. Nature 338, 741–744 (1989).
Wharton, R. P. & Struhl, G. Cell 67, 955–967 (1991).
Kobayashi, S. et al. Development 117, 885–893 (1993).
Lehmann, R. & Nüsslein-Volhard, C. Dev. Biol. 119, 402–417 (1987).
Oliver, B. et al. Genes Dev. 1, 913–923 (1987).
Warrior, R. Devl Biol. 166, 180–194 (1994).
Campos-Ortega, J. A. & Hartenstein, V. The Embryonic Development of Drosophila melanogaster. (Springer, New York, 1985).
Kobayashi, S. & Okada, M. Biotechnol. Histochem. 68, 237–239 (1993).
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Kobayashi, S., Yamada, M., Asaoka, M. et al. Essential role of the posterior morphogen nanos for germline development in Drosophila. Nature 380, 708–711 (1996). https://doi.org/10.1038/380708a0
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DOI: https://doi.org/10.1038/380708a0
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