Abstract
The ribonucleoprotein enzyme telomerase adds telomeric DNA onto chromosome ends and is normally regulated so that telomeric DNA lengths are kept within defined bounds. In the telomerase RNA gene from the yeast Kluyveromyces lactis, specific mutations that alter telomeric DNA sequences result in telomeres elongating to up to 100 times their normal length and impair cell growth. Some mutations cause immediate elongation whereas others behave like genetic time bombs, causing elongation only after a latent period of hundreds of generations.
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References
Blackburn, E. H. Cell 77, 621–623 (1994).
Blackburn, E. H. A. Rev. Biochem. 61, 113–129 (1992).
Pardue, M. L. Curr. Opin. genet. Dev. 4, 845–850 (1994).
Greider, C. W. & Blackburn, E. H. Nature 337, 331–337 (1989).
Shippen, L. D. & Blackburn, E. H. Science 247, 546–552 (1990).
Greider, C. W. Molec. cell. Biology 11, 4572–4580 (1991).
Harrington, L. A. & Greider, C. W. Nature 353, 451–454 (1991).
Lee, M. & Blackburn, E. H. Molec. cell Biol. 13, 6586–6599 (1993).
Shippen, D. E., Blackburn, E. H. & Price, C. M. Proc. natn. Acad. Sci. U.S.A. 91, 405–409 (1994).
Harley, C. B., Futcher, A. B. & Greider, C. W. Nature 345, 458–460 (1990).
Hastie, N. D. et al. Nature 346, 866–868 (1990).
Morin, G. B. Cell 59, 521–529 (1989).
Prowse, K. R., Avilion, A. A. & Greider, C. W. Proc. natn. Acad. Sci. U.S.A. 90, 1493–1497 (1993).
Shay, J. W., Wright, W. E., Brasiskyte, D. & van der Hagen, B. A. Oncogene 8, 1407–1413 (1993).
Counter, C. M., Hirte, H. W., Bacchetti, S. & Harley, C. B. Proc. natn. Acad. Sci. U.S.A. 91, 2900–2904 (1994).
Klingerhutz, A. J., Barber, S., Smith, P. P., Dyer, K. & McDougall, J. K. Molec. cell. Biol. 14, 961–969 (1994).
Blackburn, E. H. Nature 350, 569–573 (1991).
Zakian, V. A. Rev. Genet. 23, 579–604 (1989).
Shampay, J. & Blackburn, E. H. Proc. natn. Acad. Sci. U.S.A. 85, 534–538 (1988).
Runge, K. W. & Zakian, V. A. Molec cell Biol. 9, 1488–1497 (1989).
Berman, J., Tachibana, C. Y. & Tye, B.-K. Proc. natn. Acad. Sci. U.S.A. 83, 3713–3717 (1986).
Conrad, M. N., Wright, J. H., Wolf, A. J. & Zakian, V. A. Cell 63, 739–750 (1990).
Kyrion, G., Boakye, K. A. & Lustig, A. J. Molec. cell. Biol. 12, 5159–5173 (1992).
McEachern, M. J. & Blackburn, E. H. Proc. natn. Acad. Sci. U.S.A. 91, 3453–3457 (1994).
McEachern, M. J. & Hicks, J. B. Molec. cell. Biol. 13, 551–560 (1993).
Lundblad, V. & Szostak, J. W. Cell 57, 633–643 (1989).
Singer, M. S. & Gottschling, D. E. Science 266, 404–409 (1994).
Lundblad, V. & Blackburn, E. H. Cell 73, 347–360 (1993).
Yu, G.-L., Bradley, J. D., Attardi, L. D. & Blackburn, E. H. Nature 344, 126–132 (1990).
Henderson, E., Hardin, C. C., Walk, S. K., Tinoco, I. J. & Blackburn, E. H. Cell 51, 899–908 (1987).
Sundquist, W. I. & Klug, A. Nature 342, 825–829 (1989).
Veselkov, A. G., Malkov, V. A., Frank-Kamenetskii, M. D. & Dobrynin, V. N. Nature 364, 496 (1993).
Williamson, J. R. Rev. Biophys. biomol. Struct. 23, 703–730 (1994).
Gottschling, D. E. & Zakian, V. A. Cell 47, 195–205 (1986).
Price, C. M. & Cech, T. R. Biochemistry 28, 769–774 (1989).
Lustig, A. J., Kurtz, S. & Shore, D. Science 250, 549–553 (1990).
Larson, G. P., Castanotto, D., Rossi, J. J. & Malafa, M. P. Gene 150, 35–41 (1994).
Gottschling, D. E., Aparicio, O. M., Billington, B. L. & Zakian, V. A. Cell 63, 751–762 (1990).
Köhrer, K. & Domdey, H. Meth. Enzym. 194, 398–405 (1991).
Sambrook, J., Fritsch, E. F. & Maniatis, T. Molecular Cloning (Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, 1989).
Wray, L. V., Witte, M. M., Dickson, R. C. & Riley, M. I. Molec. cell Biol. 7, 1111–1121 (1987).
Sikorski, R. S. & Boeke, J. D. Meth. Enzym. 194, 302–318 (1991).
Church, G. M. & Gilbert, W. Proc. natn. Acad. Sci. U.S.A. 81, 1991–1995 (1984).
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McEachern, M., Blackburn, E. Runaway telomere elongation caused by telomerase RNA gene mutations. Nature 376, 403–409 (1995). https://doi.org/10.1038/376403a0
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DOI: https://doi.org/10.1038/376403a0
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