Abstract
The crystal structure of the RNA-binding domain of the small nuclear ribonucleoprotein U1A bound to a 21-nucleotide RNA hairpin has been determined at 1.92 Å resolution. The ten-nucleotide RNA loop binds to the surface of the β-sheet as an open structure, and the AUUGCAC sequence of the loop interacts extensively with the conserved RNP1 and RNP2 motifs and the C-terminal extension of the RNP domain. These interactions include stacking of RNA bases with aromatic side chains of proteins and many direct and water-mediated hydrogen bonds. The structure reveals the stereochemical basis for sequence-specific RNA recognition by the RNP domain.
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References
Lührmann, R., Kastner, B. & Bach, M. Biochim. biophys. Acta 1087, 265–292 (1990).
Steitz, J. A. et al. in Structure and Function of Major and Minor snRNPs (eds Birnstiel, M. L.) 115–154 (Springer, New York, 1988).
Nagai, K. & Mattaj, I. W. in RNA-protein interactions (eds Nagai, K. & Mattaj, I. W.) 150–176 (IRL, Oxford, 1994).
Sillekens, P. T. G., Habets, W. J., Beijer, R. P. & van Venrooij, W. J. EMBO J. 6, 3841–3848 (1987).
Burd, C. G. & Dreyfuss, G. Science 265, 615–621 (1994).
Birney, E., Kumar, S. & Krainer, A. Nucleic Acids Res. 21, 5803–5816 (1993).
Scherley, D. et al. EMBO J. 8, 4163–4170 (1989).
Bach, M., Krol, A. & Lührmann, R. Nucleic Acids Res. 18, 449–457 (1990).
Scherley, D., Boelens, W., Dathan, N. A., van Venrooij, W. J. & Mattaj, I. W. Nature 345, 502–506 (1990).
Nagai, K., Oubridge, C., Jessen, T.-H., Li, J. & Evans, P. R. Nature 348, 515–520 (1990).
Jessen, T.-H., Oubridge, C., Teo, C. H., Pritchard, C. & Nagai, K. EMBO J. 10, 3447–3456 (1991).
Scherley, D., Dathan, N. A., Boelens, W., van Venrooij, W. J. & Mattaj, I. W. EMBO J. 9, 3675–3681 (1990).
Lutz-Freyermuth, C., Query, C. C. & Keene, J. D. Proc. natn. Acad. Sci. U.S.A. 87, 6393–6397 (1990).
Hall, K. B. Biochemistry 33, 10076–10088 (1994).
Howe, P. W. A., Nagai, K., Neuhaus, D. & Varani, G. EMBO J. 13, 3873–3881 (1994).
Hoffmann, D. W., Query, C. C., Golden, B. L., White, S. W. & Keene, J. D. Proc. natn. Acad. Sci. U.S.A. 88, 2495–2499 (1991).
Habets, W. J. et al. Proc. natn. Acad. Sci. U.S.A. 84, 2421–2425 (1987).
Wittekind, M., Görlach, M., Friedrichs, M., Dreyfuss, G. & Mueller, L. Biochemistry 31, 6254–6265 (1992).
Görlach, M., Wittekind, M., Beckman, R. A., Mueller, L. & Dreyfuss, G. EMBO J. 11, 3289–3295 (1992).
Boelens, W. C. et al. Cell 72, 881–892 (1993).
van Gelder, C. W. G. et al. EMBO J. 12, 5191–5200 (1993).
Usman, N., Ogilvie, K. K., Tiang, M.-Y. & Cedergren, R. G. J. Am. chem. Soc. 109, 7845–7854 (1987).
Nagai, K., Evans, P. R., Li, J. & Oubridge, C. in Isomorphous Replacement and Anomalous Scattering. Proc. CCP4 St. W. (eds Wolf, W., Evans, P. R. & Leslie, A. G. W.) 141–149 (Daresbury Laboratory, Warrington, UK, 1991).
Tronrud, D. E., TenEyck, L. F. & Matthews, B. W. Acta crystallogr. A43, 489–501 (1987).
Tsai, D. E., Harper, D. S. & Keene, J. D. Nucleic Acids Res. 19, 4931–4936 (1992).
Görlach, M., Burd, C. G. & Dreyfuss, G. J. biol. Chem. 269, 23074–23078 (1994).
Query, C. C., Bentley, R. C. & Keene, J. D. Cell 57, 89–101 (1989).
Kim, Y., Geiger, J. H., Hahn, S. & Sigler, P. B. Nature 365, 512–519 (1993).
Kim, J. L., Nikolov, D. B. & Burley, S. K. Nature 365, 520–527 (1993).
Burd, C. G. & Dreyfuss, G. EMBO J. 13, 1197–1204 (1994).
Görlach, M., Burd, C. G. & Dreyfuss, G. Expl Cell Res. 211, 400–407 (1994).
Rould, M. A., Perona, J. J., Söll, D. & Steitz, T. A. Science 246, 1135–1142 (1989).
Ruff, M. et al. Science 252, 1682–1689 (1991).
Belrhali, H. et al. Science 263, 1432–1436 (1994).
Rould, M. A., Perona, J. J. & Steitz, T. A. Nature 352, 213–218 (1991).
Cavarelli, J., Rees, B., Ruff, M., Thierry, J.-C. & Moras, D. Nature 362, 181–184 (1993).
Valegård, K., Murray, J. B., Stockley, P. G., Stonehouse, N. J. & Liljas, L. Nature 371, 623–626 (1994).
Lindahl, M. et al. EMBO J. 13, 1249–1254 (1994).
Sakabe, N. Nucl. Inst. Meth. A303, 448–463 (1991).
Leslie, A. G. W. in Joint CCP4 and ESF-EACMB Newsletter on Protein Crystallography No. 26 (Daresbury Laboratory, Warrington, UK, 1992).
CCP4 suite Acta crystallogr. D50, 760–763 (1994).
Cosier, J. & Glazer, A. M. J. appl. Cryst. 19, 105–107 (1986).
Teng, T. J. appl. Crystallogr. 23, 387–391 (1990).
Sheldrick, G. M. in Isomorphous Replacement and Anomalous Scattering, Proc. CCP4 St. W. (eds Wolf, W., Evans, P. R., & Leslie, A. G. W.) 23–38 (Daresbury Laboratory, Warrington, UK, 1991).
Otwinowski, Z. in Isomorphous Replacement and Anomalous Scattering, Proc. CCP4 St. W. (eds Wolf, W., Evans, P. R. & Leslie, A. G. W.) 80–86 (Daresbury Laboratory, Warrington, UK, 1991).
Abrahams, J. P., Leslie, A. G. A., Lutter, R. & Walker, J. E. Nature 370, 621–628 (1994).
Jones, T. A., Zou, J. Y., Cowan, S. W. & Kjeldgaard, M. Acta crystallogr. A47, 110–119 (1991).
Krol, A. et al. Nucleic Acids Res. 18, 3803–3811 (1990).
Brünger, A. T. Nature 335, 472–475 (1992).
Gait, M. J., Pritchard, C. & Slim, G. in Oligonucleotide Synthesis: A Practical Approach (ed. Eckstein. F.) 25–48 (IRL, Oxford, 1991).
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Oubridge, C., Ito, N., Evans, P. et al. Crystal structure at 1.92 Å resolution of the RNA-binding domain of the U1A spliceosomal protein complexed with an RNA hairpin. Nature 372, 432–438 (1994). https://doi.org/10.1038/372432a0
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DOI: https://doi.org/10.1038/372432a0
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