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The ETS domain protein Pointed-P2 is a target of MAP kinase in the Sevenless signal transduction pathway

Nature volume 370, pages 386–389 (1994)Cite this article

Abstract

THE fate of the R7 photoreceptor cell in the developing eye of Drosophila is controlled by the Sevenless (Sev) receptor tyrosine kinase1,2. Sev activates a highly conserved signal transduction cascade involving the proteins Rasl and Raf and the Rolled/mitogen-activated protein (Rl/MAP) kinase3. Here we show that the ETS domain protein encoded by the P2 transcript of the pointed (pnt) gene is a nuclear target of this signalling cascade which acts downstream of Rl/MAP kinase. The PntP2 protein is phosphorylated by Rl/MAP kinase in vitro at a single site and this site is required for its function in vivo. Furthermore, we present genetic and biochemical data suggesting that MAP kinase controls neural development through phosphorylation of two antagonizing transcription factors of the ETS family, Van and PntP2.

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Authors and Affiliations

  1. Zoologisches Institut, Universitat Zurich, Winterthurerstrasse 190, CH-8057, Zurich, Switzerland

    Damian Brunner, Klaus Dücker, Nadja Oellers & Ernst Hafen

  2. Institut fur Entwickiungsbiologie, Universitat Koln, D-50923, Koln, Germany

    Henrike Scholzi & Christian Klambt

Authors
  1. Damian Brunner
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  2. Klaus Dücker
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  3. Nadja Oellers
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  4. Ernst Hafen
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  5. Henrike Scholzi
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  6. Christian Klambt
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Brunner, D., Dücker, K., Oellers, N. et al. The ETS domain protein Pointed-P2 is a target of MAP kinase in the Sevenless signal transduction pathway. Nature 370, 386–389 (1994). https://doi.org/10.1038/370386a0

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