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Letters to Nature
Nature 370, 386 - 389 (04 August 1994); doi:10.1038/370386a0

The ETS domain protein Pointed-P2 is a target of MAP kinase in the Sevenless signal transduction pathway

Damian Brunner*, Klaus Dücker*, Nadja Oellers*, Ernst Hafen*, Henrike Scholzi & Christian Klambt

*Zoologisches Institut, Universitat Zurich, Winterthurerstrasse 190, CH-8057 Zurich, Switzerland
Institut fur Entwickiungsbiologie, Universitat Koln, D-50923 Koln, Germany
To whom correspondence should be addressed.

THE fate of the R7 photoreceptor cell in the developing eye of Drosophila is controlled by the Sevenless (Sev) receptor tyrosine kinase1,2. Sev activates a highly conserved signal transduction cascade involving the proteins Rasl and Raf and the Rolled/mitogen-activated protein (Rl/MAP) kinase3. Here we show that the ETS domain protein encoded by the P2 transcript of the pointed (pnt) gene is a nuclear target of this signalling cascade which acts downstream of Rl/MAP kinase. The PntP2 protein is phosphorylated by Rl/MAP kinase in vitro at a single site and this site is required for its function in vivo. Furthermore, we present genetic and biochemical data suggesting that MAP kinase controls neural development through phosphorylation of two antagonizing transcription factors of the ETS family, Van and PntP2.

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