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Nature 368, 269-271 (17 March 1994) | doi:10.1038/368269a0
Multi-target PCR analysis by capillary electrophoresis and laser-induced fluorescence
Wei Lu, Dai-Shu Han, Ju Yuan & Jean-Marie Andrieu
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Abstract
Quantitative analysis of polymerase chain reaction (PCR) amplified HIV-1 DNA or cDNA fragments is attained using an automated system that combines capillary-gel electrophoresis (CGE) for high-efficiency separation and laser-induced fluorescence (LIF) for high-sensitivity detection. This system enables the detection of PCR-amplified multiple target DNA or cDNA in the same tube by a single injection with high precision.
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