Abstract
THE general transcripton factor TFIIE, together with other general transcription factors, is essential for transcription initiation by RNA polymerase II1–5. TFIIE stimulates the TFIIH-dependent kinase activity that phosphorylates the carboxy-terminal domain of the largest subunit of RNA polymerase II6, and possesses a helicase activity7. Here we show that human TFIIH has DNA-dependent ATPase activity and we characterize the stimulatory effect of TFIIE on both the ATPase and kinase activities. We demonstrate that extensive phosphorylation of RNA polymerase II occurs in a TFIIE-dependent manner in both the absence and presence of DNA but, in the latter case, only at a late stage of preinitiation complex assembly. We also show that TFIIH specifically phosphorylates three general transcription factors, human TFIIDτ (TBP), TFIIE-α and TFIIF-α (RAP74).
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Ohkuma, Y. et al. Nature 354, 398–401 (1991).
Sumimoto, H. et al. Nature 354, 401–404 (1991).
Roeder, R. G. Trends biol. Sci. 16, 402–408 (1991).
Zawal, L. & Reinberg, D. Prog. Nucleic Acid Res. molec Biol. 44, 67–108 (1993).
Inostroza, J., Flores, O. & Reinberg, D. J. biol. Chem. 266, 9304–9308 (1991).
Lu, H., Zawel, L., Fischer, L., Egly, J.-M. & Reinberg, D. Nature 358, 641–645 (1992).
Schaeffer, L. et al. Science 260, 58–63 (1993).
Fischer, L. et al. Science 257, 1392–1395 (1992).
Conaway, R. C. & Conaway, J. W. Proc. natn. Acad. Sci. U.S.A. 86, 7356–7360 (1989).
Sawadogo, M. & Roeder, R. G. J. biol. Chem. 259, 5321–5326 (1984).
Rappaport, J. & Weinmann, R. J. biol. Chem. 262, 17510–17515 (1987).
Feaver, W. J., Gileadi, O., Li, Y. & Kornberg, R. D. Cell 67, 1223–1230 (1991).
Corden, J. L., Cadena, D. L., Ahearn, J. H. & Darmus, M. E. Proc. natn. Acad. Sci. U.S.A. 82, 7934–7938 (1985).
Serizawa, H., Conaway, R. C. & Conaway, J. W. Proc. natn. Acad. Sci. U.S.A. 89, 7476–7480 (1992).
Conaway, R. C., Bradsher, J. N. & Conaway, J. W. J. biol. Chem. 267, 8464–8467 (1992).
Usheva, A. et al. Cell 69, 871–881 (1992).
Suzuki, M. Nature 344, 562–565 (1990).
Hoffmann, A. et al. Nature 346, 387–390 (1990).
Payne, J. M., Laybourn, P. J. & Dahmus, M. E. J. biol. Chem. 264, 19621–19629 (1989).
Laybourn, P. J. & Dahmus, M. E. J. biol. Chem. 265, 13165–13173 (1990).
Chesnut, J. D., Stephans, J. H. & Dahmus, M. E. J. biol. Chem. 267, 10500–10506 (1992).
Lu, H., Flores O., Weinmann, R. & Reinberg, D. Proc. natn. Acad. Sci. U.S.A. 88, 10004–10008 (1991).
Serizawa, H., Conaway, J. W. & Conaway, R. C. Nature 363, 371–374 (1993).
Wang, W., Carey, M. & Gralla, J. D. Science 255, 450–453 (1992).
Sawadogo, M. & Roeder, R. G. Proc. natn. Acad. Sci. U.S.A. 82, 4394–4398 (1985).
Sumimoto, H., Ohkuma, Y., Yamamoto, T., Horikoshi, M. & Roeder R. G. Proc. natn. Acad. Sci. U.S.A. 87, 9158–9162 (1990).
Ohkuma, Y., Sumimoto, H., Horikoshi, M. & Roeder, R. G. Proc. natn. Acad. Sci. U.S.A. 87, 9163–9167 (1990).
Seki, M. et al. Biochemistry 25, 3239–3245 (1986).
Flores, O., Lu, H. & Reinberg, D. J. biol. Chem. 267, 2786–2793 (1992).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Ohkuma, Y., Roeder, R. Regulation of TFIIH ATPase and kinase activities by TFIIE during active initiation complex formation. Nature 368, 160–163 (1994). https://doi.org/10.1038/368160a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/368160a0
This article is cited by
-
C. elegans TFIIH subunit GTF-2H5/TTDA is a non-essential transcription factor indispensable for DNA repair
Communications Biology (2021)
-
TFIIE orchestrates the recruitment of the TFIIH kinase module at promoter before release during transcription
Nature Communications (2019)
-
Structures of transcription pre-initiation complex with TFIIH and Mediator
Nature (2017)
-
Transcription initiation complex structures elucidate DNA opening
Nature (2016)
-
Structural basis of transcription initiation by RNA polymerase II
Nature Reviews Molecular Cell Biology (2015)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.
