Nature 367, 460 - 463 (03 February 1994); doi:10.1038/367460a0

Conservation of wingless patterning functions in the short-germ embryos of Tribolium castaneum

Lisa M. Nagy & Sean Carroll

Howard Hughes Medical Institute, Laboratory of Molecular Biology, University of Wisconsin, Madison, Wisconsin 53706, USA

DURING embryogenesis, all insects reach a conserved, or phylotypic, stage at which all future segments are present^1,2. Different insects, however, arrive at this stage by overtly different pathways. In the long-germ insect Drosophila melanogaster, segmentation of the entire embryo occurs nearly simultaneously and results from the action of a cascade of transcriptional regulatory factors that operate in the acellular environment of the syncytial blastoderm^3,4. In short-germ insects, segmentation occurs in an anterior-to-posterior sequence, within a cellular environment¹, and might then be dependent on intercellular signalling^5,6. To compare the molecular mechanisms of segmentation, we have isolated a homologue of the Drosophila wingless gene, a mediator of cell–cell communications^7–9, from the short-germ beetle Tribolium castaneum. The principal features of wingless expression patterns in Drosophila are conserved in Tribolium, including its early deployment in rostral and caudal domains in the blastoderm, its segmental iteration in cells immediately anterior to cells expressing the engrailed, gene and its later restriction to a ventral sector of the developing appendages.

References

1.	Sander, K. Adv. Insect Physiol. 12, 125−138 (1976).
2.	Sander, K. in Development & Evolution (eds Goodwin, B., Holder, N. & Wylie, C.) 137−161 (Cambridge Univ. Press, UK, 1983).
3.	Akam, M. Development 101, 1−22 (1987). | PubMed | ISI | ChemPort |
4.	Ingham, P. Nature 335, 25−34 (1988). | Article | PubMed | ISI | ChemPort |
5.	Patel, N. in Evolutionary Conservation of Developmental Mechanisms (ed Spradling, A.) 85−110 (Wiley-Liss, New York 1993).
6.	Akam, M. & Dawes, R. Curr. Biology 2 (8), 395−398 (1992). | ChemPort |
7.	Baker, N. Devl. Biol 125, 96−108 (1988). | ChemPort |
8.	Rijsewijk, F. et al. Cell 50, 649−657 (1987). | Article | PubMed | ISI | ChemPort |
9.	Cabrera, C. V., Alonso, M. C., Johnston, P., Phillips, R. G. & Lawrence, P. A. Cell 50, 659−663 (1987). | Article | PubMed | ChemPort |
10.	Nusse, R. & Varmus, H. E. Cell 69, 1073−1087 (1992). | Article | PubMed | ISI | ChemPort |
11.	Baker, N. EMBO 6, 1765−1773 (1987). | ChemPort |
12.	Baker, N. Development 103, 289−298 (1988). | PubMed | ISI | ChemPort |
13.	van den Heuvel, M., Nusse, R., Johnston, P. & Lawrence, P. A. Cell 59, 739−749 (1989). | Article | PubMed | ChemPort |
14.	Ingham, A. & Hidalgo, P. W. Development 117, 283−291 (1993). | PubMed | ISI | ChemPort |
15.	Sommer, R. & Tautz, D. Nature 361, 448−450 (1993). | Article | PubMed | ISI | ChemPort |
16.	Patel, N., Condron, B. & Zinn, K. Nature 367, 429−134 (1994). | Article | PubMed | ISI | ChemPort |
17.	Ingham, P. W., Baker, N. E. & Martinez-Arias, A. Nature 331, 73−75 (1988). | Article | PubMed | ISI | ChemPort |
18.	Martinez-Arias, A., Baker, N. E. & Ingham, P. W. Development 103, 157−170 (1988). | PubMed |
19.	Cohen, S. M. Nature 343, 173−175 (1990). | Article | PubMed | ISI | ChemPort |
20.	Simcox, A. A. et al. Development 107, 715−722 (1989).
21.	Struhl G. & Basler, K. Cell 72, 527−540 (1993). | Article | PubMed | ISI | ChemPort |
22.	Couso, J. P., Bate, M. & Martinez-Arias, A. Science 259, 484−489 (1993). | PubMed | ISI | ChemPort |
23.	Stuart, J. J., Brown, S., Beeman, R. W. & Dennel, R. E. Development 117, 233−243 (1993). | PubMed | ChemPort |
24.	Knipple, D. C., Seifert, E., Rosenberg, U. B., Preiss, A. & Jäckle, H. Nature 317, 40−45 (1985). | Article | PubMed | ISI | ChemPort |
25.	Gaul, U. & Jäckie, H. Cell 50, 639−647 (1987). | Article | PubMed | ISI | ChemPort |
26.	Mitchison, T. J. & Sedat, J. Devl. Biol. 99, 261−264 (1983). | ChemPort |
27.	Tautz, D. & Pfeifle, C. Chromosoma 98, 81−85 (1989). | Article | PubMed | ISI | ChemPort |
28.	Patel, N. H. Kornberg, T. B. & Goodman, C. S. Development 107, 201−212 (1989). | PubMed | ISI | ChemPort |
29.	Patel, N. H. et al. Cell 58, 955−968 (1989). | Article | PubMed | ISI | ChemPort |