Nature 366, 531 - 536 (09 December 1993); doi:10.1038/366531a0

Eukaryotic activators function during multiple steps of preinitiation complex assembly

Bob Choy & Michael R. Green

Program in Molecular Medicine, University of Massachusetts Medical Center, 373 Plantation Street, Worcester, Massachusetts 01605, USA

Eukaryotic activator proteins (activators) stimulate transcription by increasing assembly of the preinitiation complex. We have developed methods to quantify the stable assembly of general transcription factors into transcriptional complexes in response to activators. We show that activators function during at least two stages of preinitiation complex assembly: first, to recruit the general transcription factor TFIIB, and then at a second step, after TFIIB entry. It is at this second step that the TATA-box binding protein associated factors act. This step also seems to be critical for activators to stimulate transcription synergistically.

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