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Nature 359, 380-387 (1 October 1992) | doi:10.1038/359380a0; Accepted 4 September 1992

Continuum of overlapping clones spanning the entire human chromosome 21q

Ilya Chumakov*†, Philippe Rigault†, Sophie Guillou†, Pierre Ougen*, Alain Billaut*, Ghislaine Guasconi†, Patricia Gervy†, Isabelle LeGall*, Pascal Soularue*, Laurent Grinas†, Lydie Bougueleret*, Christine Bellanné-Chantelot*, Bruno Lacroix*, Emmanuel Barillot†, Philippe Gesnouin†, Stuart Pook†, Guy Vaysseix*†, Gerard Frelat‡, Annette Schmitz‡, Jean-Luc Sambucy*, Assumpcio Bosch§, Xavier Estivill§, Jean Weissenbach†parallel, Alain Vignal†, Harold Riethman, David Cox£, David Patterson**, Kathleen Gardiner**, Masahira Hattori††, Yoshiyuki Sakaki††, Hitoshi Ichikawa‡‡, Misao Ohki‡‡, Denis Le Paslier*, Roland Heilig§§, Stylianos Antonarakisparallelparallel & Daniel Cohen*†¶¶

  1. * Centre d'Etude du Polymorphisme Humain (CEPH), 27 rue Juliette Dodu, 75010 Paris, France
  2. †Genethon, 1 rue de I'lnternationale, BP 59, 91002 Evry Cedex, France
  3. ‡Centre d'Energie Atomique, 68 Avenue Division LeClerc, 92260 Fontenay aux Roses, France
  4. §Institut de Recerca Oncologica, Hospital Duran i Reynals Ctra Castelldefels, KM 2.7, Barcelona 08907, Spain
  5. parallelCNRS URA 1445, Institut Pasteur, 28 rue Docteur Roux, 75724 Paris, France
  6. The Wistar Institute, 36th Street and Spruce, Philadelphia, Pennsylvania 19104, USA
  7. £Neurogenetics Laboratory, University of California, 401 Parnassus Avenue, San Francisco, California 94143, USA
  8. **Eleanor Roosevelt Institute, 1899 Gaylord Street, Denver, Colorado 80206, USA
  9. ††The Institute of Medical Science, The University of Tokyo, 4-6-1 Shirokanedai Minatoku, Tokyo 108, Japan
  10. ‡‡Saitama Cancer Center Research Institute, Ina-machi, Kitaadachi-gun, Saitama-ken 362, Japan
  11. §§INSERM U184, Laboratoire de Génétique Moléculaire de Eucaryotes, 11 rue Humann, Strasbourg, France
  12. parallelparallelCenter for Medical Genetics, The Johns Hopkins University School of Medicine, 600 North Wolfe Street CMSC 1003, Baltimore, Maryland 21205, USA
  13. ¶¶To whom correspondence should be addressed.
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A continuous array of overlapping clones covering the entire human chromosome 21q was constructed from human yeast artificial chromosome libraries using sequence-tagged sites as landmarks specifically detected by polymerase chain reaction. The yeast artificial chromosome contiguous unit starts with pericentromeric and ends with subtelomeric loci of 21q. The resulting order of sequence-tagged sites is consistent with other physical and genetic mapping data. This set of overlapping clones will promote our knowledge of the structure of this chromosome and the function of its genes.