Nature 358, 641 - 645 (20 August 1992); doi:10.1038/358641a0

Human general transcription factor IIH phosphorylates the C-terminal domain of RNA polymerase II

Hua Lu^*, Leigh Zawel^*, Laurent Fisher^†, Jean-Marc Egly^† & Danny Reinberg^*‡

^*Department of Biochemistry, Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey, 675 Hoes Lane, Piscataway, New Jersey 08854–5635, USA
^†Laboratoire de Genetique Moleculaire (CNRS), U184 (INSERM) des Eucaryotes, Faculte de Medecine, 67085 Strasbourg, France

Phosphorylation of the carboxy-terminal domain of the largest subunit of RNA polymerase II is believed to control the transition from transcription initiation to elongation. The general transcription factor IIH (TFIIH) contains a kinase activity capable of phosphorylating this domain. Factors that promote the association of RNA polymerase II with the preinitiation complex stimulate this activity. The transcription factor HE, which is required for the stable association of TFIIH with the preinitiation complex, affects the processivity of TFIIH kinase.

References

1.	Zawel, L. & Reinberg, D. Prog. Nucleic Acid Res. molec. Biol. (in the press).
2.	Flores, O., Lu, H., Killeen, M., Greenblatt, J. & Reinberg, D. Proc. natn. Acad. Sci. U.S.A. 88, 9999−10003 (1991). | ChemPort |
3.	Maldonado, E., Ha, I., Cortes, P., Weis, L. & Reinberg, D. Molec. cell. Biol. 10, 6335−6347 (1990). | PubMed | ChemPort |
4.	Buratowski, S., Hahn, S., Fuarente, L. & Sharp, P. A. Cell 56, 549−561 (1989). | Article | PubMed | ISI | ChemPort |
5.	Corden, J. F. Trends biochem. Sci. 15, 383−387 (1990). | Article | PubMed | ChemPort |
6.	Lu, H., Flores, O., Weinmann, R. & Reinberg, D. Proc. natn. Acad. Sci. U.S.A. 88, 10004−10008 (1991). | ChemPort |
7.	Flores, O., Lu, H. & Reinberg, D. J. biol. Chem. 267, 2786−2793 (1992). | PubMed | ISI | ChemPort |
8.	Cadena, D. & Dahmus, M. J. biol. Chem. 262, 12468−12474 (1987). | PubMed | ISI | ChemPort |
9.	Payne, J. M., Laybourn, P. J. & Dahmus, M. J. biol. Chem. 264, 19621−19629 (1989). | PubMed | ISI | ChemPort |
10.	Usheva, A. et al. Cell 69, 871−881 (1992). | Article | PubMed | ISI | ChemPort |
11.	Laybourn, P. J. & Dahmus, M. J. biol. Chem. 265, 13165−13173 (1990). | PubMed | ISI | ChemPort |
12.	Cisek, L. J. & Corden, J. F. Nature 339, 679−684 (1989). | Article | PubMed | ChemPort |
13.	Cisek, L. J. & Corden, J. F. Meth. Enzym. 200, 301−325 (1991). | PubMed | ChemPort |
14.	Arias, J. A., Peterson, S. R. & Dynan, W. S. J. biol. Chem. 266, 8055−8061 (1991). | PubMed | ChemPort |
15.	Peterson, S. R., Dvir, A., Anderson, C. W. & Dynan, W. S. Genes Dev. 6, 426−438 (1992). | PubMed | ISI | ChemPort |
16.	Lee, J. M. & Greenleaf, A. L. Proc. natn. Acad. Sci. U.S.A. 86, 3624−3628 (1989). | ChemPort |
17.	Lee, J. M. & Greenleaf, A. L. Gene Expression 1, 149−162 (1991). | PubMed | ChemPort |
18.	Feaver, W., Gileadi, O., Li, Y. & Kornberg, R. D. Cell 67, 1223−1230 (1991). | Article | PubMed | ISI | ChemPort |
19.	Stone, N. & Reinberg, D. J. biol. Chem. 267, 6353−6360 (1992). | PubMed | ChemPort |
20.	Dahmus, M. & Dynan, W. S. in Transcription Regulation (eds McNight, S. L. & Yamarmoto, K.) (Cold Spring Harbor Laboratory Press, New York, in the press).
21.	Young, R. A. A. Rev. Biochem. 60, 689−715 (1991). | Article | ChemPort |
22.	Cho, K. W. Y., Khalili, K., Zandomeni, R. & Weinmann, R. J. J. biol. Chem. 260, 15204−15210 (1985). | PubMed | ChemPort |
23.	Kim, W. Y. & Dahmus, M. E. J. biol. Chem. 264, 3169−3174 (1989). | PubMed | ChemPort |
24.	Zehring, W. A., Lee, J. M., Weeks, J. R., Jokerst, R. S. & Greenleaf, A. L. Proc. natn. Acad. Sci. U.S.A. 85, 3698−3702 (1988). | ChemPort |
25.	Thompson, N. E., Aronson, D. B. & Burgess, R. R. J. biol. Chem. 265, 7069−7077 (1990). | PubMed | ISI | ChemPort |
26.	Gerard, M. et al. J. biol. Chem. 266, 20940−20945 (1991). | PubMed | ISI | ChemPort |
27.	Fischer, L. et al. Science (in the press).
28.	Dynlacht, B. D., Hoey, T. & Tjian, R. Cell 66, 563−576 (1991). | Article | PubMed | ISI | ChemPort |
29.	Cortes, P., Flores, O. & Reinberg, D. Molec. cell. Biol. 12, 413−421 (1992). | PubMed | ISI | ChemPort |
30.	Bunick, D., Zandomeni, R., Ackerman, S. & Weinmann, R. Cell 29, 877−886 (1982). | Article | PubMed | ISI | ChemPort |
31.	Conaway, J. W., Bradsher, J. N. & Conaway, R. C. J. biol. Chem. 267, 10142−10148 (1992). | PubMed | ChemPort |
32.	Conaway, R. C. & Conaway, J. W. Proc. natn. Acad. Sci. U.S.A. 86, 7356−7360 (1989). | ChemPort |
33.	Ha, I., Lane, W. & Reinberg, D. Nature 352, 689−695 (1991). | Article | PubMed | ChemPort |
34.	Peterson, M. G. et al. Nature 354, 369−373 (1991). | Article | PubMed | ISI | ChemPort |
35.	Flores, O., Ha, I. & Reinberg, D. J. biol. Chem. 265, 5629−5634 (1990). | PubMed | ChemPort |
36.	Sawadogo, M. & Roeder, R. G. Proc. natn. Acad. Sci. U.S.A. 82, 4394−4398 (1985). | ChemPort |