Nature 357, 333 - 335 (28 May 1992); doi:10.1038/357333a0

Molecular cloning of the receptor for human antidiuretic hormone

Mariel Birnbaumer, Anita Seibold, Stephanie Gilbert, Masami Ishido^*, Claude Barberis^*, Anaid Antaramian, Phillippe Brabet & Walter Rosenthal

Department of Cell Biology, Baylor College of Medicine, Houston, Texas 77030, USA
^*Present addresses: Institute of Applied Biochemistry, University of Tsukuba, Ibaraki-ken 305, Japan (M.I.); Institute CNRS-INSERM de Pharmacologie-Endocrinologie; Rue de la Cardonille, Montpellier CEDEX 34094, France (C.B.).

ANTIDIURESIS, the recovery of water from the lumen of the renal collecting tubule, is regulated by the hypothalamic release of antidiuretic hormone (ADH), which binds to specific receptors on renal collecting tubule cells, stimulates adenylyl cyclase and promotes the cyclic AMP-mediated incorporation of water pores into the luminal surface of these cells'^1–3. We report here the isolation of the human ADH receptor gene using a genomic expression cloning approach⁴. The gene was used to clone the complementary DNA from a human renal library. The deduced amino-acid sequence of the receptor yields a hydropathy profile characteristic of receptors with seven putative transmembrane regions. This and the comparison with other cloned receptors indicates that the ADH receptor is a member of the superfamily of G-protein-coupled receptors.

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