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Adaptive protein evolution at the Adh locus in Drosophila

Abstract

PROTEINS often differ in amino-acid sequence across species. This difference has evolved by the accumulation of neutral mutations by random drift, the fixation of adaptive mutations by selection, or a mixture of the two. Here we propose a simple statistical test of the neutral protein evolution hypothesis based on a comparison of the number of amino-acid replacement substitutions to synonymous substitutions in the coding region of a locus. If the observed substitutions are neutral, the ratio of replacement to synonymous fixed differences between species should be the same as the ratio of replacement to synonymous polymorphisms within species. DNA sequence data on the Adh locus (encoding alcohol dehydrogenase, EC 1.1.1.1) in three species in the Drosophila melanogaster species subgroup do not fit this expectation; instead, there are more fixed replacement differences between species than expected. We suggest that these excess replacement substitutions result from adaptive fixation of selectively advantageous mutations.

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References

  1. Sokal, R. R. & Rohlf, F. J. Biometry (Freeman, San Francisco, 1981).

    MATH  Google Scholar 

  2. Lewontin, R. C. A. Rev. Genet. 19, 81–102 (1985).

    Article  CAS  Google Scholar 

  3. Kreitman, M. & Hudson, R. R. Genetics 127, 565–582 (1991).

    CAS  PubMed  PubMed Central  Google Scholar 

  4. Nei, M. Molecular Evolutionary Genetics (Columbia University Press, New York, 1987).

    Google Scholar 

  5. Sharp, P. M. & Li, W.-H. J. molec. Evol. 28, 398–402 (1989).

    Article  ADS  CAS  Google Scholar 

  6. Kreitman, M. Nature 304, 412–417 (1983).

    Article  ADS  CAS  Google Scholar 

  7. Haldane, J. B. S. J. Genet. 57, 511–524 (1957).

    Article  Google Scholar 

  8. Maynard Smith, J. Nature 219, 1114–1116 (1968).

    Article  Google Scholar 

  9. Sved, J. A. Am. Nat. 102, 283–293 (1968).

    Article  Google Scholar 

  10. Collet, C. J. molec. Evol. 27, 142–146 (1988).

    Article  ADS  CAS  Google Scholar 

  11. Bodmer, M. & Ashburner, M. Nature 309, 425–430 (1984).

    Article  ADS  CAS  Google Scholar 

  12. Cohn, V. H. & Moore, G. P. Molec. Biol. Evol. 5, 154–166 (1988).

    CAS  PubMed  Google Scholar 

  13. Zagursky, R. J., Baumeister, K., Lomas, N. & Berman, M. L. Gene Analyt. Techn. 2, 89–94 (1985).

    Article  CAS  Google Scholar 

  14. Saiki, R. K., Bugawan, T. L., Horn, G. T., Mullis, K. B. & Erlich, H. A. Nature 324, 163–166 (1988).

    Article  ADS  Google Scholar 

  15. Higuchi, R. G. & Ochman, H. Nucleic Acids Res. 17, 5865 (1989).

    Article  CAS  Google Scholar 

Download references

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McDonald, J., Kreitman, M. Adaptive protein evolution at the Adh locus in Drosophila. Nature 351, 652–654 (1991). https://doi.org/10.1038/351652a0

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